Team:Freiburg/Notebook/20 August

From 2011.igem.org

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{{:Team:Freiburg/Templates/header}}
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/19_August">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 20 August </a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/21_August">Next entry</a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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===Trafo===
===Trafo===
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name:Rüdiger
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date:20.08
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Date 19.08. Name Rüdiger
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Experiment Ligation
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|-
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| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: Precipitator
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|}
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Procedure
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# take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
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# thaw cells on ice 20 minutes
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# pipette 50 μl cells and 2 μl DNA into eppi still on ice!
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# Incubate for 30 minutes on ice
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# Heat at 42°C for 60 sec
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# Incubate on ice for 5 minutes
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# Add 200 μl LB Broth
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# Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
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# Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
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'''Documentation:'''
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Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
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{| style="border-spacing:0;"
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Trying to get Precipitator into iGEM vector.
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Did not work.
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|}
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'''Investigators: Rüdiger'''
'''Investigators: Rüdiger'''
Transformation of precipitator(pSB1C3).
Transformation of precipitator(pSB1C3).
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Trafo did not work.
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10.09.
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Rüdiger
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Picked cells from yesterday's transformation
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1 a/b A-C
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2 a/b A-C
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3 a/b A-C
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5 a/b A-C
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6 a/b A-C
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7 a/b A-C
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-miniprep of yesterday's inoculation.
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11.09.
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-miniprep of yesterday's inoculation.
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{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 01:10, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Miniprep

Investigators: Sophie
continue from: Transformation, 19.08.11
Testdigest showed, that cloning was not succesful. Seems that the vector was only partly digested, because only vector-bands were visible on gel. I will try it again with longer digestion times.

Miniprep

Investigators: Rüdiger

Miniprep of PET.DUET_1 vector.

Trafo

Name:Rüdiger Date:20.08
Continue from Date 19.08. Name Rüdiger

Experiment Ligation

Project Name: Precipitator

Procedure


  1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
  2. thaw cells on ice 20 minutes
  3. pipette 50 μl cells and 2 μl DNA into eppi still on ice!
  4. Incubate for 30 minutes on ice
  5. Heat at 42°C for 60 sec
  6. Incubate on ice for 5 minutes
  7. Add 200 μl LB Broth
  8. Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
  9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance

Documentation:

Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.


Trying to get Precipitator into iGEM vector.

Did not work.


Investigators: Rüdiger

Transformation of precipitator(pSB1C3).

Trafo did not work.

10.09.

Rüdiger


Picked cells from yesterday's transformation


1 a/b A-C

2 a/b A-C

3 a/b A-C

5 a/b A-C

6 a/b A-C

7 a/b A-C


-miniprep of yesterday's inoculation.


11.09.

-miniprep of yesterday's inoculation.


                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/20_August"