Team:Kyoto/Project

From 2011.igem.org

(Difference between revisions)
(Prototype team page)
(Project)
 
(82 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{Kyoto_Foreground|active_page=project}}
 +
{{Kyoto_Background}}
 +
{{Kyoto_WikiDesign}}
 +
= '''Project''' =
 +
このページにはプロジェクトの概要を書いて詳細はリンク先に書くことを提案します。<br>
 +
それと各プロジェクトの名前はもう少し検討してもいいと思うのです。hunger→nutrient switchとかluminescenceも誤解を招く表現に思われます。
 +
 +
== '''Overview''' ==
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<iframe width="280" height="175" src="https://static.igem.org/mediawiki/2011/f/f0/CarnivorousEcoli.swf" frameborder="0"
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
style="float:right; padding: 3px 20px 5px 20px" allowfullscreen></iframe>
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
</html>
</html>
 +
Escherichia coli is generally not regarded as an animal because they don't hunt or eat. Thus, the creation of carnivorous E.coli which catches and digests bugs like other animals challenges this notion. Fries are baited, caught and digested, becoming a supplementary ammoniacal source when the media doesn't contain enough gultamine. The project consists of 4 minor projects.
-
<!-- *** End of the alert box *** -->
+
求訂正。論文用の書き出しなのでwikiではもっと詳しくしないといけない。そもそも実験がすべてうまくいった前提で書いてあることに注意。
-
 
+
-
{|align="justify"
+
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:Kyoto_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Kyoto_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:Kyoto | Team Example]]
+
-
|}
+
-
 
+
-
<!--- The Mission, Experiments --->
+
-
 
+
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
-
!align="center"|[[Team:Kyoto|Home]]
+
-
!align="center"|[[Team:Kyoto/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Kyoto Official Team Profile]
+
-
!align="center"|[[Team:Kyoto/Project|Project]]
+
-
!align="center"|[[Team:Kyoto/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Kyoto/Modeling|Modeling]]
+
-
!align="center"|[[Team:Kyoto/Notebook|Notebook]]
+
-
!align="center"|[[Team:Kyoto/Safety|Safety]]
+
-
!align="center"|[[Team:Kyoto/Attributions|Attributions]]
+
-
|}
+
-
 
+
-
 
+
-
 
+
-
 
+
-
== '''Overall project''' ==
+
-
 
+
-
Your abstract
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
== Project Details==
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
=== Part 2 ===
+
 +
== '''Project Details''' ==
 +
ここでは各プロジェクトのページに飛ぶリンクだけをはる予定なので、簡単な説明はhomeへ、より詳しい説明は各プロジェクトのページに記載するようにお願いします。by Kato
-
=== The Experiments ===
+
=== '''<html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Team Hunger</a></html>''' ===
 +
Nutritional switch is constructed by the genes from glnA operon.
 +
=== '''<html><a href="https://2011.igem.org/Team:Kyoto/Luminescence">Team Luminescence</a></html>''' ←「Project Capture」とかの方が適切かもしれませんね。。。by草場===
 +
We demonstrated the strong phototaxis to ultraviolet and weaker phototaxis to lights of other wavelength.
 +
<html><a href="https://2011.igem.org/Team:Kyoto/Luminescence/Introduction">Introduction</a>  made by 草場  ※要添削<br></html>
 +
In nature, some of organisms which eat insects lure them. Rafflesia attract flies by distinctive smell. ''Pyrearinus termitilluminans'', which makes and lives in tunnel in anthill of termite ''Cornitermes cumulans'', emits light in the first week of rainy season to hunt the termites. ''Arachnocampa'' also uses light for hunting flies.
 +
For Carnivorous E.coli, we chose light as the method to lure insects like ''Pyrearinus termitilluminans'', because it is simple and there is an available biobrick which has the function to emit light: BBa_K3225909 created by iGEM 2010 Cambridge Team.<br>
-
=== Part 3 ===
+
To probe that Carnivorous E.coli can lure insects. we, group luminescence, intend to do the following things.
 +
*Confirmation that drosophila, our model organism, have positive phototaxis in blue light (E.glowli created by 2010 Cambridge Team emits blue light)
 +
*Confirmation that drosophila moves to light emitted by E.coli
 +
<br><br>
 +
<html><a href="https://2011.igem.org/Team:Kyoto/Luminescence/Note">Notebook</a><br></html>
 +
=== '''<html><a href="https://2011.igem.org/Team:Kyoto/Digestion">Team Digestion</a></html>''' ===
 +
Chitin, the main component of exoskeleton of fry is degraded by ChitinaseA1 and protein which composes the body is degraded by SAM-P20, serine protease. The exocrine of ChitinaseA1 and SAM-P20 was measured both quantitatively and qualitatively.<br>
 +
Our goal is following two things
 +
*Quantitative and qualitative Measurement of ChiA1 and SAM-P20
-
== Results ==
+
目標:大腸菌から消化酵素(プロテアーゼ、キチナーゼ)を分泌させ、実際にハエを溶かす。

Latest revision as of 09:01, 4 October 2011

Contents

Project

このページにはプロジェクトの概要を書いて詳細はリンク先に書くことを提案します。
それと各プロジェクトの名前はもう少し検討してもいいと思うのです。hunger→nutrient switchとかluminescenceも誤解を招く表現に思われます。

Overview

Escherichia coli is generally not regarded as an animal because they don't hunt or eat. Thus, the creation of carnivorous E.coli which catches and digests bugs like other animals challenges this notion. Fries are baited, caught and digested, becoming a supplementary ammoniacal source when the media doesn't contain enough gultamine. The project consists of 4 minor projects.

求訂正。論文用の書き出しなのでwikiではもっと詳しくしないといけない。そもそも実験がすべてうまくいった前提で書いてあることに注意。


Project Details

ここでは各プロジェクトのページに飛ぶリンクだけをはる予定なので、簡単な説明はhomeへ、より詳しい説明は各プロジェクトのページに記載するようにお願いします。by Kato

Team Hunger

Nutritional switch is constructed by the genes from glnA operon.

Team Luminescence ←「Project Capture」とかの方が適切かもしれませんね。。。by草場

We demonstrated the strong phototaxis to ultraviolet and weaker phototaxis to lights of other wavelength.

Introduction made by 草場 ※要添削

In nature, some of organisms which eat insects lure them. Rafflesia attract flies by distinctive smell. Pyrearinus termitilluminans, which makes and lives in tunnel in anthill of termite Cornitermes cumulans, emits light in the first week of rainy season to hunt the termites. Arachnocampa also uses light for hunting flies. For Carnivorous E.coli, we chose light as the method to lure insects like Pyrearinus termitilluminans, because it is simple and there is an available biobrick which has the function to emit light: BBa_K3225909 created by iGEM 2010 Cambridge Team.

To probe that Carnivorous E.coli can lure insects. we, group luminescence, intend to do the following things.

  • Confirmation that drosophila, our model organism, have positive phototaxis in blue light (E.glowli created by 2010 Cambridge Team emits blue light)
  • Confirmation that drosophila moves to light emitted by E.coli



Notebook

Team Digestion

Chitin, the main component of exoskeleton of fry is degraded by ChitinaseA1 and protein which composes the body is degraded by SAM-P20, serine protease. The exocrine of ChitinaseA1 and SAM-P20 was measured both quantitatively and qualitatively.

Our goal is following two things

  • Quantitative and qualitative Measurement of ChiA1 and SAM-P20

目標:大腸菌から消化酵素(プロテアーゼ、キチナーゼ)を分泌させ、実際にハエを溶かす。