From 2011.igem.org
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| - | Mini-Preps (How-To)
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| - | | + | File:May26June6GDS.jpg |
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| - | 1. Spin 1.5 mL of overnight culture for 30 secs. in micro-centrifuge.
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| - | 2. Aspirate off all but 200uL of supernatant and resuspend the pellet by vortexing.
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| - | 3. Add 300uL of TENS and mix by inversion. The solution should become viscous.
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| - | 4. Add 150uL of sodium acetate and vortex. A fine white precipitate should form.
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| - | 5. Centrifuge for 2.5 minutes.
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| - | 6. Transfer the supernatant to clean tube and add 2 volumes (1mL) of room temp ETOH.
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| - | 7. Mix and pellet DNA by centrifugation for 2-5 min.
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| - | 8. Wash pellet with 70% ethanol and allow pellet to dry.
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| - | 9. Resuspend the pellet in 30uL TE. 10. Digest 5-10uL as usual.
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| - | :PNICE--5/12/11
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| - | :PNICE (tKAN)--5/23/11
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| - | :J04430--5/23/11
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| - | :J04430--5/12/11
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| - | :Trans 003-W1--5/12/11
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| - | :Trans 003-W1--5/23/11
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| - | P.P. G.E. S.D.
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Latest revision as of 15:11, 24 September 2011
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