Team:DTU-Denmark-2

From 2011.igem.org

(Difference between revisions)

Latest revision as of 23:10, 21 September 2011

Making Molecular Biology Easier

We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.

A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.

Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.

Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.

Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.

Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.

The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page

Sponsored by

Comments or questions to the team? Please Email us

@@ Line 3: / Line 3: @@
 <head>
 <style type="text/css">
+div.flash
+{
+display: block;
+    margin-left: auto;
+    margin-right: auto;
+left:0px;
+top:0px;
+z-index:-200;
+}
+ul.dropdown * {
+         z-index: 200;
+}
     div.image {
      padding-right: 10px;
-     padding-left: 50px;
+     padding-left: 10px;
      padding-bottom:10px;
      padding-top:10px;
-     float: right;
+     text-align:center;
    }
+ div.image2 {
+    display: block;
+    margin-left: auto;
+    margin-right: auto;
+  }
    div.slogan {
-   padding-right: 30px;
+   padding-right: 20px;
-     padding-left: 30px;
+     padding-left: 20px;
-     padding-bottom:30px;
+     padding-bottom:20px;
-     padding-top:30px;
+     padding-top:20px;
      background-color:#FBFF74;
      border-style: bold;
@@ Line 29: / Line 51: @@
 }
+div.igemlogo img {
-  div.igemlogo img {
      margin: 20px 20px 20px 20px;
          }
+ div.red {
+    padding-right: 30px;
+    padding-left: 30px;
+    padding-bottom:30px;
+    padding-top:30px;
+    text-align:center;
+    background-color:#E7F9FF
+}
+ div.grey {
+    padding-right: 5px;
+    padding-left: 5px;
+    padding-bottom:5px;
+    padding-top:5px;
+    width: 260px;
+    height: 1150px;
+    background-color:#F9F9F9
+  }
+ div.green {
+    padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width: 189px;
+    height: 220px;
+    background-color:#D8F6CE;
+    float:left;
+    margin:5px;
+    line-height:120%;
+}
+ div.blue {
+    padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width: 189px;
+    height: 220px;
+    line-height:120%;
+       background-color:#FFDEB6;
+float:left;
+margin:5px;
+}
+ div.yellow {
+    padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width: 189px;
+    height: 220px;
+    float:left;
+    margin:5px;
+line-height:120%;
+    background-color:#F6CED8;
+}
+div.purple {
+   padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width:  189px;
+    height:  220px;
+    float:left;
+    margin:5px;
+    line-height:120%;
+    background-color:#A9E2F3;
+}
+div.lightgreen {
+   padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width:  189px;
+    height:  220px;
+    line-height:120%;
+ float:left;
+    margin:5px;
+background-color:#FAFEBA;
+}
+div.pink {
+    padding-right: 10px;
+    padding-left: 10px;
+    padding-bottom:10px;
+    padding-top:10px;
+    width:  189px;
+    height:  220px;
+    line-height:120%;
+ float:left;
+    margin:5px;
+    background-color:#CEF6E3;
+}
 <!--
 A{text-decoration:none}
-A:link {color:  #990000;text-decoration: none }
+A:link {color:  #0000FF;text-decoration: none }
-A:active {color:  #990000;text-decoration: none }
+A:active {color:  #0000FF;text-decoration: none }
-A:visited {color:  #990000 ;text-decoration: none }
+A:visited {color:  #0000FF ;text-decoration: none }
 A:hover {text-decoration: none}
 -->
@@ Line 52: / Line 164: @@
 <!-- Main content area -->
-<table cellpadding=20px>
+<table cellpadding=10px>
-<object width="400" height="400" data="https://static.igem.org/mediawiki/2011/d/d8/Flash-anim-2.swf"></object>
+<td width="660px" height="100%" valign="top" >
+<div class="flash">
+<object type="application/x-shockwave-flash" width="600" height="400" data="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf">
+<param name="movie" value="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf")>
+<param name="wmode" value="transparent">
+</object>
-  <td width="600px" height="100%" valign="top" >
+</div>
-  <font color="#990000" face="arial" size="6" >
-<p align="center">
+<div class="red">
-<b>What Plug 'n' Play is all about</b><br>
+<p align="justify">
+<font color="#000000" face="arial" size="5" >
+<b>Making Molecular Biology Easier</b>
+</font></p>
+<font color="#000000" face="arial" size="2" >
+<p align="justify">
+<br>
+<p align="justify">
+<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
 </p>
-  </font>
+</font>
+</div>
+ <br>
+<div class="green">
+<font color="#000000" face="arial" size="3" >
+<b>A Reporter System</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/8/84/IGEM_001.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
 <p align="justify">
+As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
+</font>
+</div>
-<img src="https://static.igem.org/mediawiki/2011/1/12/Overveiw_4_bred_4.png" height="500px" align="center"> </img>
+<div class="blue">
+<font color="#000000" face="arial" size="3" >
+<b>Flexibility</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/a/aa/Plasmid_puzzle_colour.jpg" width="187px" height="50px"> </img><br>
-  <td width="182px" height="100%" valign="top" class="box">
+</div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
+</font>
+</div>
+<div class="yellow">
+<font color="#000000" face="arial" size="3" >
+<b>Applications</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/8/8f/Application.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
+</font>
+</div>
+<br>
+<br>
+<div class="purple">
+<font color="#000000" face="arial" size="3" >
+<b>Data Page</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
+</font>
+</div>
+<div class="lightgreen">
+<font color="#000000" face="arial" size="3" >
+<b>Achievements</b><br><br>
+</font>
+<div class="image2">
+<img src="https://static.igem.org/mediawiki/2011/f/f7/Achievements.jpg" width="187px" height="50px"> </img><br>
+</div>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
+</font>
+</div>
+<div class="pink">
+<font color="#000000" face="arial" size="3" >
+<b>The Team</b><br><br>
+</font>
+<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
+<font color="#000000" face="arial" size="1" >
+<p align="justify">
+We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
+</font>
+</div>
+  <td width="182px" height="100%" valign="top" >
+<div class="grey">
+<br>
+<div class="image">
+<font color="#990000" face="arial" size="3" >
+<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
+<a href="https://2011.igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/3/3f/Igem.png
+" height="120px" align="Center">  </a>
+</font>
+</div>
+<br>
+<br>
+<div id="fb-root"></div>
+<script>(function(d, s, id) {
+  var js, fjs = d.getElementsByTagName(s)[0];
+  if (d.getElementById(id)) {return;}
+  js = d.createElement(s); js.id = id;
+  js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
+  fjs.parentNode.insertBefore(js, fjs);
+}(document, 'script', 'facebook-jssdk'));</script>
+<div class="fb-like-box" data-href="https://www.facebook.com/pages/DTU-iGEM-Team-2011/217344154973456" data-width="250" data-show-faces="true" data-stream="true" data-header="true"></div>
+<br>
+<br>
+<br>
 <div class="slogan">
 <span id="slogan"></span>
@@ Line 88: / Line 353: @@
    slogan[9] = "Plug’n’play with DNA will change the world today";
    slogan[10] = "Plug’n’play can hold your favorite DNA";
+  slogan[11] = "Mix it, heat it, use it, it's that easy";
+  slogan[12] = "Avoid restriction, use the plug 'n' play edition";
+  slogan[13] = "Make a smart decision, use the plug 'n' play edition";
+  slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
    var rand = Math.floor(Math.random()*slogan.length);
    $('#slogan').text("\"" + slogan[rand] + "\"");
@@ Line 94: / Line 364: @@
 </div>
-<br>
-<br>
-<br>
-<div class="image">
-<font color="#990000" face="arial" size="3" >
-<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
-<a href="https://2011.igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/3/3f/Igem.png
-" height="120px" align="Center">  </a>
-</font>
 </div>
 <br>
 <br>
+ </td>
+</table>
-<iframe src="http://www.facebook.com/plugins/likebox.php?href=https%3A%2F%2Fwww.facebook.com%2Fpages%2FDTU-iGEM-Team-2011%2F217344154973456&amp;width=292&amp;colorscheme=light&amp;show_faces=true&amp;border_color&amp;stream=true&amp;header=true&amp;height=427" scrolling="no" frameborder="0" style="border:none; overflow:hidden; width:292px; height:427px;" allowTransparency="true"></iframe>
-<br>
-  </font>
 <br>
 <br>
+<table>
- </td>
-</table>
-<table>
 <tr height="25px">
-<td width="967px" valign="center" align="center">
+<td width="976px" valign="center" align="center">
-   <font color="#990000" face="arial" size="6">
+   <font color="#000000" face="arial" size="5">
 <b> Sponsored by</b><br><br>
 </font>
@@ Line 136: / Line 384: @@
 <img src="https://static.igem.org/mediawiki/2011/a/a2/Novozymes.jpeg" with="150px"height="150px"> </img>&nbsp;
 <img src="https://static.igem.org/mediawiki/2011/5/5c/Bn_logo_rgb.jpg" with="260px"height="260px"> </img> <br><br>
+<img src="https://static.igem.org/mediawiki/2009/d/de/Ottomfond.jpg" with="230px"height="65px"> </img>&nbsp;
+<img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"height="40px"> </img> <br><br>
 <img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
 <img src="https://static.igem.org/mediawiki/2011/7/74/Novo_nordisk_logo.jpg" with="150px"height="150px"> </img> &nbsp;