Team:Lyon-INSA-ENS/Realisation/Protocols

From 2011.igem.org

(Difference between revisions)
 
(30 intermediate revisions not shown)
Line 3: Line 3:
{{INSA-Lyon/styletestaurelie}}
{{INSA-Lyon/styletestaurelie}}
{{Lyon-INSA-ENS/menuhorizontal}}
{{Lyon-INSA-ENS/menuhorizontal}}
-
{{Lyon-INSA-ENS/menuNotebookProtocoles}}
+
<!--{{Lyon-INSA-ENS/menuNotebookProtocoles}}-->
 +
{{Lyon-INSA-ENS/menuNotebookVertical|Protocols = actif}}
<html>
<html>
 +
 +
<div style="float : left; margin-top : -10px; margin-left : -200px">
 +
  <a href="https://2011.igem.org/Main_Page" >
 +
      <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
 +
  </a>
 +
</div>
<body>
<body>
Line 16: Line 23:
      
      
     <br/> <br/>
     <br/> <br/>
-
     <br/><br/><br/><br/><br/>
+
     <br/> <br>
         <p> <font color="green" size="5">
         <p> <font color="green" size="5">
Line 24: Line 31:
<br> <br><br> <br>
<br> <br><br> <br>
-
<p style=" line-height : 1.5em"> Antibiotics were used at the following concentrations : <br>
+
<p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br><br>
Ampicilin (Amp) : 100µg/mL <br>
Ampicilin (Amp) : 100µg/mL <br>
Chloramphenicol (Cm) : 20µg/mL <br>
Chloramphenicol (Cm) : 20µg/mL <br>
Spectinomycin (Spc) : 100µg/mL <br>
Spectinomycin (Spc) : 100µg/mL <br>
Kanamycin (Kan) : 30µg/mL<br>
Kanamycin (Kan) : 30µg/mL<br>
-
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br>
+
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br></p>
-
The composition of the different media we have used is the following :<br>
+
<p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br>
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br>
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br>
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br>
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br>
Line 44: Line 51:
      
      
     <br/> <br/>
     <br/> <br/>
-
     <br/><br/><br/><br/><br/>
+
     <br/><br/><br/>
         <p> <font color="green" size="5">
         <p> <font color="green" size="5">
Line 52: Line 59:
     <br> <br>
     <br> <br>
 +
    <br/> <br/>
 +
All the protocols can be downloaded in pdf version by clicking on the corresponding logo :
     <br/> <br/>
     <br/> <br/>
<p> .  
<p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a>  
 +
<a href=" https://static.igem.org/mediawiki/2011/3/36/Biofilm_quantification.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
   <p> .  
   <p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a>  
 +
<a href="https://static.igem.org/mediawiki/2011/f/fd/CaCl2_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
   <p> .  
   <p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a>  
 +
<a href="https://static.igem.org/mediawiki/2011/6/65/Fermentas_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
  <p> .  
  <p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a>
 +
<a href="https://static.igem.org/mediawiki/2011/9/9d/Ligation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
   <p> .  
   <p> .  
-
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli </a>
+
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a>
 +
<a href="https://static.igem.org/mediawiki/2011/4/4f/NucleoSpin_Plasmid_QuickPure.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
 +
  </p>
 +
 
 +
    <br/> <br/><br/>
 +
 
 +
<p>.
 +
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy"> Microscopy test </a>
 +
<a href="https://static.igem.org/mediawiki/2011/d/d5/Microscopy_Test.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
 +
<br/>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
<p> .
<p> .
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a>
 +
<a href="https://static.igem.org/mediawiki/2011/f/f8/Ozyme_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
     <br/> <br/>
     <br/> <br/>
-
    <br/> <br/>
+
 +
 
-
  <p> .
+
<p> .  
-
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits </a>  
+
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a>
 +
<a href="https://static.igem.org/mediawiki/2011/7/7f/Pouring_of_plates.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
-
  <p> .  
+
<p> .
-
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> Plasmid DNA purification using QIAprep® Spin Miniprep Kit</a>
+
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a>
 +
<a href="https://static.igem.org/mediawiki/2011/5/53/QUIGen_midiprep_kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
-
 
+
  <p> .  
-
<p> .  
+
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a>
-
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a>
+
<a href="https://static.igem.org/mediawiki/2011/6/67/QUIGen_Miniprep_Kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
<p> .  
<p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a>
 +
<a href="https://static.igem.org/mediawiki/2011/2/2a/Storage_of_strains.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
   </p>
   </p>
-
    <br/> <br/>
 
     <br/> <br/>
     <br/> <br/>
     <p> .
     <p> .
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a>
 +
<a href="https://static.igem.org/mediawiki/2011/c/c7/TSS_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
     </p>
     </p>
      
      
 +
<br><br><br><br><br><br><br>
 +
 +
    <p>
 +
              <a  href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/BeforeStarting"/><font color="grey"><b>Before Starting</b></font></a>
 +
              <a  style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/Week1"/><font color="grey"><b>First Week</b></font></a>
 +
              <br/>
 +
    </p>
 +
<br><br><br>
</div>
</div>
</body>
</body>

Latest revision as of 16:08, 20 September 2011







General culture conditions





Antibiotics were used at the following concentrations :

Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :

LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water









Choose one of our protocols to read its description





All the protocols can be downloaded in pdf version by clicking on the corresponding logo :

. Biofilm quantification



. CaCl2 chemical transformation



. Fermentas digestion



. Ligation



. Macherey-Nagel miniprep kit




. Microscopy test



. Ozyme digestion



. Pouring of plates



. QIAGen midiprep kit



. QIAGen miniprep kit



. Storage of strains



. TSS chemical transformation








Before Starting First Week








ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Protocols"