"
Team:Lyon-INSA-ENS/Realisation/Protocols
From 2011.igem.org
(Difference between revisions)
| (31 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
{{INSA-Lyon/styletestaurelie}} | {{INSA-Lyon/styletestaurelie}} | ||
{{Lyon-INSA-ENS/menuhorizontal}} | {{Lyon-INSA-ENS/menuhorizontal}} | ||
| - | {{Lyon-INSA-ENS/menuNotebookProtocoles}} | + | <!--{{Lyon-INSA-ENS/menuNotebookProtocoles}}--> |
| + | {{Lyon-INSA-ENS/menuNotebookVertical|Protocols = actif}} | ||
<html> | <html> | ||
| + | |||
| + | <div style="float : left; margin-top : -10px; margin-left : -200px"> | ||
| + | <a href="https://2011.igem.org/Main_Page" > | ||
| + | <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" /> | ||
| + | </a> | ||
| + | </div> | ||
<body> | <body> | ||
| Line 16: | Line 23: | ||
<br/> <br/> | <br/> <br/> | ||
| - | <br/><br | + | <br/> <br> |
<p> <font color="green" size="5"> | <p> <font color="green" size="5"> | ||
| Line 24: | Line 31: | ||
<br> <br><br> <br> | <br> <br><br> <br> | ||
| - | <p style=" line-height : 1.5em"> Antibiotics were used at the following concentrations : <br> | + | <p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br><br> |
Ampicilin (Amp) : 100µg/mL <br> | Ampicilin (Amp) : 100µg/mL <br> | ||
Chloramphenicol (Cm) : 20µg/mL <br> | Chloramphenicol (Cm) : 20µg/mL <br> | ||
Spectinomycin (Spc) : 100µg/mL <br> | Spectinomycin (Spc) : 100µg/mL <br> | ||
Kanamycin (Kan) : 30µg/mL<br> | Kanamycin (Kan) : 30µg/mL<br> | ||
| - | The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br> | + | The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br></p> |
| - | The composition of the different media we have used is the following :<br> | + | <p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br> |
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br> | LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br> | ||
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br> | M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br> | ||
| Line 39: | Line 46: | ||
<br> | <br> | ||
<br/> <br/> | <br/> <br/> | ||
| + | </div> | ||
<div class="contenugrand2";> | <div class="contenugrand2";> | ||
<br/> <br/> | <br/> <br/> | ||
| - | + | <br/><br/><br/> | |
<p> <font color="green" size="5"> | <p> <font color="green" size="5"> | ||
| Line 51: | Line 59: | ||
<br> <br> | <br> <br> | ||
| + | <br/> <br/> | ||
| + | All the protocols can be downloaded in pdf version by clicking on the corresponding logo : | ||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a> | ||
| + | <a href=" https://static.igem.org/mediawiki/2011/3/36/Biofilm_quantification.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/f/fd/CaCl2_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/6/65/Fermentas_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/9/9d/Ligation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a> |
| + | <a href="https://static.igem.org/mediawiki/2011/4/4f/NucleoSpin_Plasmid_QuickPure.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
| + | </p> | ||
| + | |||
| + | <br/> <br/><br/> | ||
| + | |||
| + | <p>. | ||
| + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy"> Microscopy test </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/d/d5/Microscopy_Test.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
| + | <br/> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/f/f8/Ozyme_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
<br/> <br/> | <br/> <br/> | ||
| - | + | ||
| - | + | ||
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/ | + | <p> . |
| + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/7/7f/Pouring_of_plates.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
| - | + | <p> . | |
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/ | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a> |
| + | <a href="https://static.igem.org/mediawiki/2011/5/53/QUIGen_midiprep_kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
| - | + | <p> . | |
| - | <p> . | + | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a> |
| - | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/ | + | <a href="https://static.igem.org/mediawiki/2011/6/67/QUIGen_Miniprep_Kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> |
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/2/2a/Storage_of_strains.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| - | |||
<br/> <br/> | <br/> <br/> | ||
<p> . | <p> . | ||
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a> | <a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a> | ||
| + | <a href="https://static.igem.org/mediawiki/2011/c/c7/TSS_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br> | ||
</p> | </p> | ||
| + | <br><br><br><br><br><br><br> | ||
| + | |||
| + | <p> | ||
| + | <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/BeforeStarting"/><font color="grey"><b>Before Starting</b></font></a> | ||
| + | <a style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/Week1"/><font color="grey"><b>First Week</b></font></a> | ||
| + | <br/> | ||
| + | </p> | ||
| + | <br><br><br> | ||
</div> | </div> | ||
</body> | </body> | ||
Latest revision as of 16:08, 20 September 2011
General culture conditions
Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.
The composition of the different media we have used is the following :
LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water
Choose one of our protocols to read its description
All the protocols can be downloaded in pdf version by clicking on the corresponding logo :
.
CaCl2 chemical transformation
.
Ligation
