Team:Lyon-INSA-ENS/Realisation/Protocols

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              General culture conditions<br><HR>
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<p style=" line-height : 1.5em"><b> Antibiotics were used at the following concentrations : </b><br><br>
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Ampicilin (Amp) : 100µg/mL <br>
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Chloramphenicol (Cm) : 20µg/mL <br>
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Spectinomycin (Spc) : 100µg/mL <br>
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Kanamycin (Kan) : 30µg/mL<br>
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The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br></p>
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<p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br>
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LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.<br>
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M63G : 100/1/1 (v/v) of M63, glucose 20% and LB<br>
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LB/2 : 50/50 (v/v) mix of LB and water<br>
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All the protocols can be downloaded in pdf version by clicking on the corresponding logo :
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<p> .  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/BiofilmQuant"> Biofilm quantification </a>  
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<a href=" https://static.igem.org/mediawiki/2011/3/36/Biofilm_quantification.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/CaCl2_trans"> CaCl2 chemical transformation </a>  
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<a href="https://static.igem.org/mediawiki/2011/f/fd/CaCl2_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a>  
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas"> Fermentas digestion </a>  
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<a href="https://static.igem.org/mediawiki/2011/6/65/Fermentas_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ligation"> Ligation </a>
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<a href="https://static.igem.org/mediawiki/2011/9/9d/Ligation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli </a>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation"> Macherey-Nagel miniprep kit </a>
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<a href="https://static.igem.org/mediawiki/2011/4/4f/NucleoSpin_Plasmid_QuickPure.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<p>.
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy"> Microscopy test </a>
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<a href="https://static.igem.org/mediawiki/2011/d/d5/Microscopy_Test.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme"> Ozyme digestion</a>
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<a href="https://static.igem.org/mediawiki/2011/f/f8/Ozyme_digestion.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits </a>  
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<p> .  
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a>
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<a href="https://static.igem.org/mediawiki/2011/7/7f/Pouring_of_plates.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> Plasmid DNA purification using QIAprep® Spin Miniprep Kit</a>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification"> QIAGen midiprep kit </a>
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<a href="https://static.igem.org/mediawiki/2011/5/53/QUIGen_midiprep_kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep"> QIAGen miniprep kit</a>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Pouring"> Pouring of plates</a>
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<a href="https://static.igem.org/mediawiki/2011/6/67/QUIGen_Miniprep_Kit.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/Storage"> Storage of strains</a>
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<a href="https://static.igem.org/mediawiki/2011/2/2a/Storage_of_strains.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a>
<a href="/Team:Lyon-INSA-ENS/Realisation/Protocols/TSS_trans"> TSS chemical transformation </a>
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<a href="https://static.igem.org/mediawiki/2011/c/c7/TSS_chemical_transformation.pdf"><img src="https://static.igem.org/mediawiki/2011/6/64/Fileicon-pdf.png" style="width:25px";/></a><br>
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              <a  href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/BeforeStarting"/><font color="grey"><b>Before Starting</b></font></a>
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              <a  style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/Week1"/><font color="grey"><b>First Week</b></font></a>
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Latest revision as of 16:08, 20 September 2011







General culture conditions





Antibiotics were used at the following concentrations :

Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :

LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water









Choose one of our protocols to read its description





All the protocols can be downloaded in pdf version by clicking on the corresponding logo :

. Biofilm quantification



. CaCl2 chemical transformation



. Fermentas digestion



. Ligation



. Macherey-Nagel miniprep kit




. Microscopy test



. Ozyme digestion



. Pouring of plates



. QIAGen midiprep kit



. QIAGen miniprep kit



. Storage of strains



. TSS chemical transformation








Before Starting First Week








ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Protocols"