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From 2011.igem.org

Revision as of 20:50, 6 September 2011 (view source) Pjpatel (Talk | contribs) ← Older edit		Latest revision as of 15:10, 24 September 2011 (view source) Sallen (Talk | contribs)
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-	Created TENS	+	<gallery widths=910px heights=800px>
-	:4.5 mL TE and	+	File:May26June6GDS.jpg
-	::250uL 10% SDS	+	</gallery>
-	::250uL 2N NaOH	+
-		+
-		+
-		+
-	:-Spin 4 tubes (1.5mL) 30"	+
-	:-Pour off sol'n & resuspend	+
-	:-Add	+
-	::300uL TENS:    mix	+
-	::150uL NaOH:    mix	+
-	:-Spin 2.5 min.	+
-	:-Transfer supernatant to clean tube and add 1mL of EtOH	+
-	:-Mix and pellet DNA by centrifugation for 2-5 min	+
-	:-Wash pellet with 70% ethanol and allow pellet to dry	+
-	:-Resuspend the pellet in 30&lambda; of the TE	+
-	:-Digest 5-10&lambda;	+

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Latest revision as of 15:10, 24 September 2011