Team:Uppsala-Sweden

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<span class="hidden">Welcome to Uppsala-SwedeniGEM '2011</span>
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<div class="grid_1 link selflink">[[Team:Uppsala-Sweden | <span class="selflink">Home</span>]]</div><div class="grid_2 link">[[Team:Uppsala-Sweden/Project | Project ]]</div><div class="grid_2 link">[[Team:Uppsala-Sweden/Team | Team]]</div><div class="grid_2 link">[[Team:Uppsala-Sweden/Tools | <span class="red">Tools</span>]]</div><div class="grid_2 link">[[Team:Team:Uppsala-Sweden/Notebook | Notebook]]</div>
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<span class="title">''Uppsala '' <span class="bold">University</span>.</span>
 
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Our project is about ...... !
 
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=== About our project ===
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  Regulation of gene expression by light is a milestone in synthetic biology. This rapidly developing field has attracted lots of attention in the recent years. Light regulation introduces noninvasive, direct and advanced spatiotemporal control of engineered biological systems. The aim of this project is a continuation of developing the above mentioned regulation method.In 2005, the world’s first light-sensing bacteria, “coliroids”, were engineered by scientists at UT Austin. Since then, as more and more naturally occurring light-sensing microorganisms are being discovered and sequenced, synthetic biologists realize there is a whole range of natural light-sensing systems at their disposal. Most of the light-sensing systems developed thus far focus on studying one light-sensing system at a time, characterizing its activation light spectra, active state, etc. There has been a lack of focus on building light-sensing systems that sense multiple wavelengths, until very recently. The ultimate objective is to introduce control of gene expression with multiple light wavelengths and demonstrate multidimensional light control as well as fine tunability of this system by making the engineered bacteria exhibit image based on three basic colors
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Abstract
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Show color with color. The aim is to control gene expression by using only light. Our light sensing system is unique. The output modules will, when right sensor is active, express a colorfull picture.[[Team:Uppsala-Sweden/Project |(More)]]
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Team
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The team consists of 18 enthusiastic undergraduate students from the Masters Programme in Molecular Biotechnology Engineering and Bioinformatics. Together we will represent ....[[Team:Uppsala-Sweden/Team|(More)]]
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Assembly plan
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The assembly plan shows the approach of our project. It is divided in seven parts (red output, blue output, green/yellow output, chromophore, red sensor, blue sensor and green sensor). [[Team:Uppsala-Sweden/Assembly_plan|(More)]]
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                <img src="https://static.igem.org/mediawiki/2011/e/ec/B19_UU.jpg" title="Green, Red & Yellow"/>
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                <img src="https://static.igem.org/mediawiki/2011/b/b1/B29_UU.jpg" title="''Pure PCR-products to be sequenced''"/>
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                <img src="https://static.igem.org/mediawiki/2011/2/28/MG_6276.jpeg" title="Photo by Mikael Wallerstedt for the magazine Universen"/>
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                <img src="https://static.igem.org/mediawiki/2011/b/b3/MG_6291.jpeg" title="Photo by Mikael Wallerstedt for the magazine Universen"/>
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Hello for more updated information about Uppsala IGEM-2011 you can vist to [http://twitter.com/#!/iGEM2011Uppsala/ twitter] and [https://www.facebook.com/home.php?sk=group_161774083862057/ facebook]
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About our project
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<!--=== About our project ===
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you can also check our previous IGEM projects-Uppsala  https://2009.igem.org/Team:Uppsala-Sweden and https://2010.igem.org/Team:Uppsala-Sweden
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The aim of our project is to regulate gene expression by three different wavelengths and further on build a bacterial system that will act like a bacterial photocopier. With this project we will participate in the International Genetically Engineered Machine competition (iGEM).<br><br>
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The experimental part of this project is to further develop the light induced regulation of gene expression, aka coliroids. We start out by employing the principles explained by Tabor, Levskaya and Voigt's research on multichromatic light sensing. The first step is make DNA constructs for at least three light-sensing systems, each with different activation wavelengths and different outputs. Then we test each light-sensing system individually in a bacteria platform. Once this is done, we plan to express the three light-sensing systems in the same platform, at the same time and test their abilities when put together. The laboratory methods include for instance BioBrick cloning, site-directed mutagenesis, Lambda Red recombineering, etc.<br><br>
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The ultimate goal is making a "multichromatic coliroid system" capable of independent regulation of three sets of genes by light regulation. The proof of concept will be demonstrated by growing a colorful picture on an agar plate. <br><br>
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Besides this, we will also put a lot of efforts into other parts of the project including characterization of parts, marketing, human practice biosecurity, bio-ethics and website.<br><br>
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=== Human Practices ===
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You can read more about our system design at our data page:
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https://2011.igem.org/Team:Uppsala-Sweden/Project/Data
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== Blue Sensor - Animated Example ==
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=== Last info ===
 
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=== Our sponsors ===
 
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Latest revision as of 22:14, 28 October 2011

Visit our blog:

USblog.png










http://igem2011uu.blogspot.com



Abstract

Show color with color. The aim is to control gene expression by using only light. Our light sensing system is unique. The output modules will, when right sensor is active, express a colorfull picture.(More)

Team

The team consists of 18 enthusiastic undergraduate students from the Masters Programme in Molecular Biotechnology Engineering and Bioinformatics. Together we will represent ....(More)

Assembly plan

The assembly plan shows the approach of our project. It is divided in seven parts (red output, blue output, green/yellow output, chromophore, red sensor, blue sensor and green sensor). (More)




About our project

The aim of our project is to regulate gene expression by three different wavelengths and further on build a bacterial system that will act like a bacterial photocopier. With this project we will participate in the International Genetically Engineered Machine competition (iGEM).

The experimental part of this project is to further develop the light induced regulation of gene expression, aka coliroids. We start out by employing the principles explained by Tabor, Levskaya and Voigt's research on multichromatic light sensing. The first step is make DNA constructs for at least three light-sensing systems, each with different activation wavelengths and different outputs. Then we test each light-sensing system individually in a bacteria platform. Once this is done, we plan to express the three light-sensing systems in the same platform, at the same time and test their abilities when put together. The laboratory methods include for instance BioBrick cloning, site-directed mutagenesis, Lambda Red recombineering, etc.

The ultimate goal is making a "multichromatic coliroid system" capable of independent regulation of three sets of genes by light regulation. The proof of concept will be demonstrated by growing a colorful picture on an agar plate.

Besides this, we will also put a lot of efforts into other parts of the project including characterization of parts, marketing, human practice biosecurity, bio-ethics and website.

You can read more about our system design at our data page:

https://2011.igem.org/Team:Uppsala-Sweden/Project/Data


Blue Sensor - Animated Example






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