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-	-Spin 1.5 mL of overnight culture in microfuge	+	<gallery widths=910px heights=800px>
		+	File:June7GDS.jpg
		+	</gallery>
-	-Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing	+	<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:gelpicnewerGDS.jpg|425px]]
-		+	</div>
-	-Add 300uL of TENS and mix by inversion	+
-		+
-	-Add 150uL of sodium acetate and vortex	+
-		+
-	-Centrifuge for 2.5 minutes at 10K</br>	+
-		+
-	-Transfer supernatant to clean tube and add 1mL of room temp. ETOH	+
-		+
-	-Mix and pellet DNA by centrifuge for 2-5 min. at 10K	+
-		+
-	-Wash pellet with 70% ethanol and allow pellet to dry	+
-		+
-	-Resuspend pellet in 30uL of TE w/ RNA seA	+
-		+
-	-Digest 5-10uL as usual	+
-		+
-		+
-	:To make TENS, add:	+
-	::-4.5mL of TE	+
-	:::-250uL 10%SDS	+
-	:::-250uL 2N NaOH	+

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Latest revision as of 20:43, 28 September 2011