Team:UQ-Australia/Parts

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|rowspan="2"|The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.
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Here we demonstrate a biological clock being standardised as a set of BioBrick parts.
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Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.
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We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the [https://2011.igem.org/Team:UQ-Australia/Project '''Project page'''].
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! [[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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<!--- The Mission, Experiments --->
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===<span style="color:#558822"> Parts </span>===
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:UQ-Australia|Home]]
 
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!align="center"|[[Team:UQ-Australia/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UQ-Australia Official Team Profile]
 
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!align="center"|[[Team:UQ-Australia/Project|Project]]
 
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!align="center"|[[Team:UQ-Australia/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:UQ-Australia/Modeling|Modeling]]
 
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!align="center"|[[Team:UQ-Australia/Notebook|Notebook]]
 
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!align="center"|[[Team:UQ-Australia/Safety|Safety]]
 
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!align="center"|[[Team:UQ-Australia/Attributions|Attributions]]
 
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|width="100"|[[File:MultiplePromoter image.png|100x100px|link=https://2011.igem.org/File:Promoter_image.png#filelinks]]
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|width="250"|'''<span style="color:#D4A017">Plac/ara</span>'''
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===Parts===
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A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter
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|width="100"|[[File:Gene image.png|100x100px]]
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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|width="250"|'''<span style="color:#D4A017">glnG</span>'''
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Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter
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<groupparts>iGEM010 UQ-Australia</groupparts>
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|[[File:Gene image.png|100x100px]]
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|'''<span style="color:#D4A017">araC</span>'''
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Encodes for a positive regulatory protein for L-arabinose utilization in E.coli
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|[[File:PositivePromoter.png|100x100px]]
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|'''<span style="color:#D4A017">pBAD</span>'''
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L-arabinose inducible promoter
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|[[File:Gene image.png|100x100px]]
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|'''<span style="color:#D4A017">LacI</span>'''
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DNA-binding transcriptional inhibitor for lac-operon
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|[[File:PositivePromoter.png|100x100px]]
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|'''<span style="color:#D4A017">GlnAp2</span>'''
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Promoter driven by phosphorylated GlnG(NR1)
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Latest revision as of 03:58, 6 October 2011




The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.

Here we demonstrate a biological clock being standardised as a set of BioBrick parts.

Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the Project page.

UQ-Australia logo 2011.png

Parts

MultiplePromoter image.png Plac/ara

A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter

Gene image.png glnG

Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter

Gene image.png araC

Encodes for a positive regulatory protein for L-arabinose utilization in E.coli

PositivePromoter.png pBAD

L-arabinose inducible promoter

Gene image.png LacI

DNA-binding transcriptional inhibitor for lac-operon

PositivePromoter.png GlnAp2

Promoter driven by phosphorylated GlnG(NR1)

Retrieved from "http://2011.igem.org/Team:UQ-Australia/Parts"