Team:ITESM Mexico

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Using previously developed mechanisms, this project will develop a new system capable of detecting a desired analyte, the system will report the concentration of such analyte at a given time lapse with the possibility of setting an instant concentration reading at will. Since most of the mechanisms used are based in detecting arabinose, this project will use arabinose as the measured analyte to avoid unnecessary difficulties.
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Our project is a biosensor for arabinose build in an ''E. coli'' chassis, it will measure the concentration levels of arabinose by reporting two different fluorescent proteins, GFP for a low concentration level and CFP for a high concentration level.
 
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Based on the work of the [https://2009.igem.org/Team:Tokyo-Nokogen Tokyo-NokoGen (2009)] project, [https://2009.igem.org/Team:Chiba Chiba University (2010)], [https://2010.igem.org/Team:UNAM-Genomics_Mexico UNAM-Genomics México (2010)], [https://2009.igem.org/Team:British_Columbia British Columbia (2009)], [https://2010.igem.org/Team:Cambridge Cambridge (2010)] and [https://2010.igem.org/Team:Tec-Monterrey ITESM campus Monterrey (2010)] we fused two ideas: creating a sensor that will measure the concentration level at out command and it will be reset-able. This will allow us to obtain a concentration reading of arabinose whenever we want it, improving the current lock-and-key mechanism. It will also include a master switch mechanism that works with a biological photosensor included in our design. Exposing our bacterial culture to green light will activate this mechanism, switching the biosensor on.
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::'''We all know that the International Genetically Engineered Machine competition is the premiere undergraduate Synthetic Biology competition.''''
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::Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells.
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::'''iGEM goal:''' “Design, build and test biological systems done from interchangeable biological standard parts”.
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::We could imagine that when the first planners of this contest started this idea, they never thought that this competition will became the most important for undergraduate genetic engineering students from the whole world. ::We can observe in the following graph the exponential growth of the attendance.  
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::As one of the few Mexican teams that have taken the challenge of attending to this competition, we will create a summary of the Mexican participation on this space:  
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::*The first Mexican team to attend IGEM was the “IGEM Mexico” team (Such a creative name!) in 2006. The team was formed by UNAM and IPN students.  
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::*LCG-UNAM-Mexico participated on  iGEM 2008
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::*Then the UNAM went by itself in 2009  
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::*In 2010 UNAM won a gold medal with  “WiFi Coli: A Communicolight System
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::*ITESM Campus Monterrey 2010 also won a medal the same year
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::*In 2011 there are a lot more Mexican groups in the competition:  ITESM CQ, ITESM MTY, UANL and UNAM
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::We would like take this chance to tell our story. We are the first generation of biotechnology engineers of ITESM CQ, and in consequence we also were the first group in the campus to try to attend to iGEM.
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::At the beginning we thought that the main concern was to think in a cool iGEM project, but that was only the first step of the road. After several months discussing ideas, we decided to take the well known path of building a biosensor. When we finished up the theoretical construct, we relized that we would need a lot of effort to get the resources, so when we started obtaining founds we thought that now it was just matter of ordering the strains and reagents, but we could have not imagined the trial that awaited us.
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The aim of this project is to design three bimolecular mechanisms that will enable the organism to 1) Detect the presence of a desired analyte (arabinose) and give a color response accordingly to its concentration. 2) Determine the concentration at regular time intervals 3) Respond to a non-chemical signal to give an immediate reading of the concentration.
 
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The results will be obtained by reading the amount of light with a flux-cytometer. This way we will not only obtain a concentration range but a precise concentration reading.
 
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In the future this biosensor will work as a chassis to detect a wide variety of compounds such as toxins and/or antibodies.
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[[Team:ITESM_Mexico/Team Roster]]
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[[Team:ITESM_Mexico/Track Selection]]
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[[Team:ITESM_Mexico/Overview]]
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[[Team:ITESM_Mexico/Diagram pathway]]
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[[Team:ITESM_Mexico/Introduction]]
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[[Team:ITESM_Mexico/In need of solution]]
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[[Team:ITESM_Mexico/Background]]
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[[Team:ITESM_Mexico/Objectives]]
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[[Team:ITESM_Mexico/Expected results]]
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[[Team:ITESM_Mexico/Future researh]]
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[[Team:ITESM_Mexico/Research labs and groups]]
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[[Team:ITESM_Mexico/Gallery]]
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[[Team:ITESM_Mexico/Construct]]
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[[Team:ITESM_Mexico/Ethical considerations]]
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[[Team:ITESM_Mexico/Impact]]
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[[Team:ITESM_Mexico/Abstract]]
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[[Team:ITESM_Mexico/About iGEM]]
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[[Team:ITESM_Mexico/Experimental design]]
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[[Team:ITESM_Mexico/Photo Gallery]]
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[[Team:ITESM_Mexico/Bitacora]]
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[[Team:ITESM_Mexico/Future research]]
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[[Team:ITESM_Mexico/Results]]
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[[Team:ITESM_Mexico/Index]]
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[[Team:ITESM_Mexico/Glossary]]
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[[Team:ITESM_Mexico/Interactive Tour]]
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[[Team:ITESM_Mexico/Abbreviations]]
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[[Team:ITESM_Mexico/Acknowledgments]]
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Latest revision as of 03:12, 29 September 2011

ITESM MÉXICO

SensE.coli

Igem Itesm

Home


Click on BioBrickCoatl to Begin


We all know that the International Genetically Engineered Machine competition is the premiere undergraduate Synthetic Biology competition.'
Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells.
iGEM goal: “Design, build and test biological systems done from interchangeable biological standard parts”.
We could imagine that when the first planners of this contest started this idea, they never thought that this competition will became the most important for undergraduate genetic engineering students from the whole world. ::We can observe in the following graph the exponential growth of the attendance.
As one of the few Mexican teams that have taken the challenge of attending to this competition, we will create a summary of the Mexican participation on this space:
  • The first Mexican team to attend IGEM was the “IGEM Mexico” team (Such a creative name!) in 2006. The team was formed by UNAM and IPN students.
  • LCG-UNAM-Mexico participated on iGEM 2008
  • Then the UNAM went by itself in 2009
  • In 2010 UNAM won a gold medal with “WiFi Coli: A Communicolight System
  • ITESM Campus Monterrey 2010 also won a medal the same year
  • In 2011 there are a lot more Mexican groups in the competition: ITESM CQ, ITESM MTY, UANL and UNAM
We would like take this chance to tell our story. We are the first generation of biotechnology engineers of ITESM CQ, and in consequence we also were the first group in the campus to try to attend to iGEM.
At the beginning we thought that the main concern was to think in a cool iGEM project, but that was only the first step of the road. After several months discussing ideas, we decided to take the well known path of building a biosensor. When we finished up the theoretical construct, we relized that we would need a lot of effort to get the resources, so when we started obtaining founds we thought that now it was just matter of ordering the strains and reagents, but we could have not imagined the trial that awaited us.


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