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Team:Warsaw/Safety
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| - | <div | + | <h2>Safety</h2> |
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| + | <div class="note">Would any of your project ideas raise safety issues in terms of researcher safety</div> | ||
| + | <div align="justify"> | ||
| + | During our project we mainly use our cell-free cloning procedure. It requires only work with DNA. No GMO organisms are produced. GMO organisms are produced at final stage - when the final constructs are transformed to the cells. | ||
| + | We work only with <i>E. coli</i> non-pathogenic strain. Except Ampicilin and Chloramphenicol resistance genes there are no potentially dangerous biobricks in our constructs. | ||
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| + | <br> | ||
| - | + | Work in a microbiology lab environment:<br> | |
| - | + | In the lab there is work with GMO and <i>E. coli</i> bacteria strains. | |
| - | + | Researchers have to wear lab coats and gloves. | |
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| - | Work in a microbiology lab | + | |
| - | In the lab there is work with GMO and E. | + | |
| - | Researchers have to wear lab coats and gloves | + | |
Work with toxic chemical compounds:<br> | Work with toxic chemical compounds:<br> | ||
| - | Ethidium Bromide can cause cancer - is used only to visualize gels | + | Ethidium Bromide can cause cancer - is used only to visualize gels |
It is not added to agarose gels before running the gel.<br> | It is not added to agarose gels before running the gel.<br> | ||
Antibiotics: Chloramphenicol and Ampicillin:<br> | Antibiotics: Chloramphenicol and Ampicillin:<br> | ||
| - | While making antibiotics solutions from the powder the mask | + | While making antibiotics solutions from the powder the mask |
| - | and safety glasses have to be worn<br> | + | and safety glasses have to be worn.<br> |
Work in the RNAse free environment:<br> | Work in the RNAse free environment:<br> | ||
| - | Hazards include RNAse cleaners like RNA zap, a mask should be worn. | + | Hazards include RNAse cleaners like RNA zap, a mask should be worn. |
| - | The cleaners are irritating<br> | + | The cleaners are irritating.<br> |
Work with UV light:<br> | Work with UV light:<br> | ||
| - | UV light is a cause of cancer therefore a UV protective mask, gloves | + | UV light is a cause of cancer therefore a UV protective mask, gloves |
| - | and lab coats have to be worn while working with the UV light<br> | + | and lab coats have to be worn while working with the UV light.<br> |
| + | <div class="note"> public safety, or environmental safety? </div> | ||
| + | <div align="justify"> | ||
| + | The biobrick that we create are not dangerous to the environment. However the spread of the Chloramphenicol resistance gene present in biobrick vector would generate Chloramphenicol resistance bacteria and increasing pool of the antibiothic resistance genes in the environment. | ||
| + | Therefore the microbiological wastes should be properly disposed - as adviced by the university | ||
| + | to ensure that no GMO organisms or toxic compounds gets to the environment. | ||
| + | </div> | ||
| + | <div class="note"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</div> | ||
| + | <div align="justify"> | ||
| + | No, currently our planned BioBricks are completely biosafe. | ||
| + | We use only constructs expressing fluorescent proteins. | ||
| + | The new brick includes modified RBS parts. | ||
| + | To our best knowledge those bricks are safe to use in the lab.<br> | ||
| + | In case of release to the environment those biobrick are not toxic or do not cause pathogenicity or are not a source of evolutionary advantage.</div> | ||
| - | < | + | <div class="note">Is there a local biosafety group, committee, or review board at your institution? </div> |
| - | + | <div align="justify"> | |
| - | + | In Poland there are no biosafety groups locally at the universities. | |
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We are following Biosafety guidelines of Polish Ministry of Environment. | We are following Biosafety guidelines of Polish Ministry of Environment. | ||
| - | + | Our department also obtained the necessary permission to work with GMO organisms. Therefore it is fully legal to engineer <i>E. coli</i> according to Polish law.</div> | |
| - | + | ||
| - | < | + | <div class="note"> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </div> |
| - | </ | + | <div align="justify"> |
| - | </ | + | We designed a protocol to perform cloning without use of living organisms. |
| - | </html> | + | This protocol allows cloning biobricks without the need to produce GMO organisms. |
| + | Therefore according to Polish law and biosafety rules it allows even high schools to take part in iGEM. | ||
| + | The Genetically Modified Organism is created only at the final stage - to test the device. | ||
| + | It can be performed at a GMO certified institution.<br /></div> | ||
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Latest revision as of 20:37, 21 September 2011
Safety
Would any of your project ideas raise safety issues in terms of researcher safety
During our project we mainly use our cell-free cloning procedure. It requires only work with DNA. No GMO organisms are produced. GMO organisms are produced at final stage - when the final constructs are transformed to the cells.
We work only with E. coli non-pathogenic strain. Except Ampicilin and Chloramphenicol resistance genes there are no potentially dangerous biobricks in our constructs.
Work in a microbiology lab environment:
In the lab there is work with GMO and E. coli bacteria strains. Researchers have to wear lab coats and gloves. Work with toxic chemical compounds:
Ethidium Bromide can cause cancer - is used only to visualize gels It is not added to agarose gels before running the gel.
Antibiotics: Chloramphenicol and Ampicillin:
While making antibiotics solutions from the powder the mask and safety glasses have to be worn.
Work in the RNAse free environment:
Hazards include RNAse cleaners like RNA zap, a mask should be worn. The cleaners are irritating.
Work with UV light:
UV light is a cause of cancer therefore a UV protective mask, gloves and lab coats have to be worn while working with the UV light.
public safety, or environmental safety?
The biobrick that we create are not dangerous to the environment. However the spread of the Chloramphenicol resistance gene present in biobrick vector would generate Chloramphenicol resistance bacteria and increasing pool of the antibiothic resistance genes in the environment.
Therefore the microbiological wastes should be properly disposed - as adviced by the university
to ensure that no GMO organisms or toxic compounds gets to the environment.
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
No, currently our planned BioBricks are completely biosafe.
We use only constructs expressing fluorescent proteins.
The new brick includes modified RBS parts.
To our best knowledge those bricks are safe to use in the lab.
In case of release to the environment those biobrick are not toxic or do not cause pathogenicity or are not a source of evolutionary advantage.
In case of release to the environment those biobrick are not toxic or do not cause pathogenicity or are not a source of evolutionary advantage.
Is there a local biosafety group, committee, or review board at your institution?
In Poland there are no biosafety groups locally at the universities.
We are following Biosafety guidelines of Polish Ministry of Environment.
Our department also obtained the necessary permission to work with GMO organisms. Therefore it is fully legal to engineer E. coli according to Polish law.
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
We designed a protocol to perform cloning without use of living organisms.
This protocol allows cloning biobricks without the need to produce GMO organisms.
Therefore according to Polish law and biosafety rules it allows even high schools to take part in iGEM.
The Genetically Modified Organism is created only at the final stage - to test the device.
It can be performed at a GMO certified institution.
