Team:Freiburg/Notebook/3 September

From 2011.igem.org

(Difference between revisions)
(→3A-assembly:Digestion)
(→3A-assembly:Digestion)
Line 9: Line 9:
Digestion of:
Digestion of:
-
InsertI:
+
'''InsertI:'''
*64
*64
*46  
*46  
Line 15: Line 15:
with EcorI and SpeI
with EcorI and SpeI
-
InsertII:
+
'''InsertII:'''
*67
*67
*50
*50

Revision as of 11:51, 3 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

InsertI:

  • 64
  • 46

with EcorI and SpeI

InsertII:

  • 67
  • 50

with XbaI and PstI

Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hours.

blue light receptor

Stop of ligation

Investigators: Sandra

Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.

To do: Trafo

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Picking clones

Investigators: Sandra

Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/3_September"