Team:Freiburg/Notebook/3 September

From 2011.igem.org

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(→Picking clones)
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===3A-assembly:Digestion===
-
'''Investigators:NAME'''
+
'''Investigators: Sandra'''
 +
Digestion of:
 +
InsertI:
 +
*64
 +
*46
 +
 +
with EcorI and SpeI
 +
 +
InsertII:
 +
*67
 +
*50
 +
 +
with XbaI and PstI
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==

Revision as of 11:50, 3 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

3A-assembly:Digestion

Investigators: Sandra

Digestion of:

InsertI:

  • 64
  • 46

with EcorI and SpeI

InsertII:

  • 67
  • 50

with XbaI and PstI

blue light receptor

Stop of ligation

Investigators: Sandra

Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.

To do: Trafo

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Picking clones

Investigators: Sandra

Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.

                       

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