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Team:UTP-Panama/Project
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| + | THERMOGENIC RESPONSE NUTRIENT BIOSENSOR (THE REMBO) | ||
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Develop flexible and better sensor for environmental, agricultural and engineering applications is the aims of the UTP-Panama Team “SynBio Engineering Tool kit”. In this way we work with the Nitrate Biosensor (PyeaR - GFP composite) developed by Team BCCS-Bristol 2010, which expresses fluorescent signals upon nutrient detection, producing a high-resolution map of arable land. To achieve this goal we use the collateral effect of the AOX enzyme (Alternative oxidase) mainly designed to generate heat in response to a cold-shock, using the hybB promoter. This effect was the rising in bacterial growth under 20°C. | Develop flexible and better sensor for environmental, agricultural and engineering applications is the aims of the UTP-Panama Team “SynBio Engineering Tool kit”. In this way we work with the Nitrate Biosensor (PyeaR - GFP composite) developed by Team BCCS-Bristol 2010, which expresses fluorescent signals upon nutrient detection, producing a high-resolution map of arable land. To achieve this goal we use the collateral effect of the AOX enzyme (Alternative oxidase) mainly designed to generate heat in response to a cold-shock, using the hybB promoter. This effect was the rising in bacterial growth under 20°C. | ||
Finally we design an prototype device with a better cold shock promoter (CspA promoter) develop by UNAM-CINVESTAV 2010 iGEM Team, to improve the heat generation and AOX expression at low temperatures. | Finally we design an prototype device with a better cold shock promoter (CspA promoter) develop by UNAM-CINVESTAV 2010 iGEM Team, to improve the heat generation and AOX expression at low temperatures. | ||
Revision as of 04:06, 3 September 2011
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TitleTHERMOGENIC RESPONSE NUTRIENT BIOSENSOR (THE REMBO) ABSTRACTDevelop flexible and better sensor for environmental, agricultural and engineering applications is the aims of the UTP-Panama Team “SynBio Engineering Tool kit”. In this way we work with the Nitrate Biosensor (PyeaR - GFP composite) developed by Team BCCS-Bristol 2010, which expresses fluorescent signals upon nutrient detection, producing a high-resolution map of arable land. To achieve this goal we use the collateral effect of the AOX enzyme (Alternative oxidase) mainly designed to generate heat in response to a cold-shock, using the hybB promoter. This effect was the rising in bacterial growth under 20°C. Finally we design an prototype device with a better cold shock promoter (CspA promoter) develop by UNAM-CINVESTAV 2010 iGEM Team, to improve the heat generation and AOX expression at low temperatures.
The orignal idea: July 15Traditional Engineering fields as Electrical, Mechanical, Civil, Agricultural, etc. have been working for last two centuries separately from Biological develops (life sciences). The UTP-Panama Team is decided to brake this paradigm in the Republic of Panama. To accomplish this goal, the UTP-Panama Team is working to develop what we call "'SynBio Engineering Tool Kit", the manufacturing of engineering tools obtained from biological resources. At this moment, Engineering groups are designing projects in different fields, using the biological devices to improve and solve traditional engineering issues. In this sense, the proposed works are: Agricultural & Environmental Engineering • Nitrogen Area: introduce chemotaxis to BioBrick BBa_K381001 develop by the Bristol 2010 iGEM Team, to make a dynamics Nitrate-nitrite sensor. The main goal of this area (future) is to express LegHemoglobine and other nitrogen fixation molecules in E.Coli. • Phosphates sensing: BBa_K116401 & BBa_K116404. • pH sensing: Taiwan BacToKidney pH sensors BBa_K116001 & BBa_K116002 Mechanical & Material Sciences Engineering • Biopolimer Area: continue the Virgina (2008), Utah State (2009) and Insa-Lyon 2010 develop in the field of Bioplastic-Biopolymers through expression genes for PHA, PHA, and PHC. • Heat Production: uses and improvement of Gatech Biobrick BBa_K410000, on mechanical engineering. Electrical Engineering • Biosensing • Light Sensitive proteins: work with BBa_K191003 • Creation of electrical circuits Project DetailsPart 2The ExperimentsPart 3== Results == |
