Team:UT Dallas/NotebookWeek3

From 2011.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 412: Line 412:
         <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li>
         <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li>
         <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li>
         <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li>
-
         <li><a href="https://2011.igem.org/Team:UT_Dallas/Gallery"><font size="3" face="verdana">Gallery</a></font></li>
+
         <li><a href="https://2011.igem.org/Team:UT_Dallas/Safety"><font size="3" face="verdana">Safety</a></font></li>
          
          
       </ul>
       </ul>
Line 428: Line 428:
         <div class="article">
         <div class="article">
           <h2><span> Week 3</span></h2>
           <h2><span> Week 3</span></h2>
-
           <p> <font size="3" face="verdana"></font></p>
+
           <p><font size = "5" face= "arial" color = "#184D79"><b>July 25-</b></font>
 +
              <blockquote><li type = "circle">Incubate LB chlora for 1 hour<li type = "circle">Negative control continued overnight<li type = "circle">Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.</blockquote>
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 28-</b></font>
 +
              <blockquote><li type = "circle">i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate <li type = "circle">Incubation FOFR colonies (3 mL of LB and carb)<li type = "circle">Plating mutated CheZ (inoculation)<li type = "circle">grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB<li type = "circle">-grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB</blockquote>
 +
 
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 29-</b></font>
 +
              <blockquote><li type = "circle">Making glycerol stock<li type = "circle">Miniprep<li type = "circle">Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow<li type = "circle">Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity</blockquote>
 +
 
 +
        <p><font size = "5" face= "arial" color = "#184D79"><b>July 31-</b></font>
 +
              <blockquote><li type = "circle">Incubation of CheZ (I2IT)- 3mL of LB and carb</blockquote>
 +
 
 +
       
           <div class="clr"></div>
           <div class="clr"></div>
Line 477: Line 488:
         </div></a>
         </div></a>
                       <div class="gadget">
                       <div class="gadget">
-
          <h2 class="grey"><span>Multimedia</span></h2>
+
       
           <div class="clr"></div>
           <div class="clr"></div>
           <div class="testi">
           <div class="testi">
-
            <p><span class="q"><img src="https://static.igem.org/mediawiki/2011/8/85/Quote_1.gif" width="16" height="14" alt="" /></span><script src="http://widgets.twimg.com/j/2/widget.js"></script>Twitter embed
+
         
-
<script>
+
-
</script><span class="q"><img src="https://static.igem.org/mediawiki/2011/a/a1/Quote_2.gif" width="16" height="14" alt="" /></span></p>
+
Line 498: Line 507:
" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
     <div class="col c2">
     <div class="col c2">
-
       <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
+
       <h2><span><font size="5" face="arial">UT Dallas</span></font></h2>
     <img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT />     
     <img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT />     
  <p>
  <p>
Line 504: Line 513:
     </div>
     </div>
     <div class="col c3">
     <div class="col c3">
-
       <h2><span><font size="5" face="verdana">Notebook</span></font></h2>
+
       <h2><span><font size="5" face="arial">Notebook</span></font></h2>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>
       <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p>

Latest revision as of 02:12, 3 September 2011

biz solution

Week 3

July 25-

  • Incubate LB chlora for 1 hour
  • Negative control continued overnight
  • Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.

    • July 28-

  • i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate
  • Incubation FOFR colonies (3 mL of LB and carb)
  • Plating mutated CheZ (inoculation)
  • grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB
  • -grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB

    • July 29-

  • Making glycerol stock
  • Miniprep
  • Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow
  • Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity

    • July 31-

  • Incubation of CheZ (I2IT)- 3mL of LB and carb

    • Weeks 1-12

    Image Gallery

    UT Dallas

    Click here to learn more  about UTD

    Notebook

    Learn more...

    Retrieved from "http://2011.igem.org/Team:UT_Dallas/NotebookWeek3"