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Team:UT Dallas/NotebookWeek3
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<li><a href="https://2011.igem.org/Team:UT_Dallas"><font size="3" face="verdana">Home</a></font></li> | <li><a href="https://2011.igem.org/Team:UT_Dallas"><font size="3" face="verdana">Home</a></font></li> | ||
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<li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li> | <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li> | ||
<li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li> | <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li> | ||
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<h2><span> Week 3</span></h2> | <h2><span> Week 3</span></h2> | ||
| - | <p> <font size="3" face=" | + | <p><font size = "5" face= "arial" color = "#184D79"><b>July 25-</b></font> |
| + | <blockquote><li type = "circle">Incubate LB chlora for 1 hour<li type = "circle">Negative control continued overnight<li type = "circle">Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.</blockquote> | ||
| + | <p><font size = "5" face= "arial" color = "#184D79"><b>July 28-</b></font> | ||
| + | <blockquote><li type = "circle">i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate <li type = "circle">Incubation FOFR colonies (3 mL of LB and carb)<li type = "circle">Plating mutated CheZ (inoculation)<li type = "circle">grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB<li type = "circle">-grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB</blockquote> | ||
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| + | <p><font size = "5" face= "arial" color = "#184D79"><b>July 29-</b></font> | ||
| + | <blockquote><li type = "circle">Making glycerol stock<li type = "circle">Miniprep<li type = "circle">Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow<li type = "circle">Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity</blockquote> | ||
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| + | <p><font size = "5" face= "arial" color = "#184D79"><b>July 31-</b></font> | ||
| + | <blockquote><li type = "circle">Incubation of CheZ (I2IT)- 3mL of LB and carb</blockquote> | ||
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" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div> | " width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div> | ||
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| - | <h2><span><font size="5" face=" | + | <h2><span><font size="5" face="arial">UT Dallas</span></font></h2> |
<img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT /> | <img src="https://static.igem.org/mediawiki/2011/a/ad/Nserl_Pic.png" width="112" height="138" alt="" ALIGN = RIGHT /> | ||
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| - | <h2><span><font size="5" face=" | + | <h2><span><font size="5" face="arial">Notebook</span></font></h2> |
<p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p> | <p></p><p> <a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Learn more...</a></font></p> | ||
Latest revision as of 02:12, 3 September 2011
UTDallas iGem
Weeks 1-12
