Team:Tsinghua/Notebook/26 August 2011

From 2011.igem.org

(Difference between revisions)
 
Line 13: Line 13:
   sequencing result: correct
   sequencing result: correct
-
 
   freeze 2 tubes of bacteria
   freeze 2 tubes of bacteria
-
 
   mini pre. 2 tubes.
   mini pre. 2 tubes.
-
 
   --[[User:Zhu liyuan|Zhu liyuan]] 01:36, 2 September 2011 (CDT)
   --[[User:Zhu liyuan|Zhu liyuan]] 01:36, 2 September 2011 (CDT)

Latest revision as of 08:15, 2 September 2011

pro-rich mcherry parts

 pcr proline-rich mcherry again
 digest with NEB XbaI&SpeI
 final conc. 55ng/ul
 ligate pSB1C3&pro-rich mcherry
 transform
 plate
 --Zora 01:36, 2 September 2011 (CDT)

OmpA-GFP parts

 sequencing result: correct
 freeze 2 tubes of bacteria
 mini pre. 2 tubes.
 --Zhu liyuan 01:36, 2 September 2011 (CDT)