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Team:Tsinghua/Notebook/1 September 2011
From 2011.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
draw line | draw line | ||
pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate | pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate | ||
| - | --[[User:Zora|Zora]] 02: | + | --[[User:Zora|Zora]] 02:54, 2 September 2011 (CDT) |
[[new plasmid]] | [[new plasmid]] | ||
| - | + | digest | |
| - | + | ||
pSB1C3 5ug | pSB1C3 5ug | ||
digest with NEB EcoRI&SpeI | digest with NEB EcoRI&SpeI | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | + | [[pet15a - OHS parts ]] | |
| - | + | [[new plasmid]] | |
| + | gel analysis | ||
| + | OmpA-HIV cleavage site-Sh3 digested done by ZLY | ||
| + | pSB1C3 digested | ||
| + | [[File:I2011-09-01_OHS&pSB1C3_digest.jpg]] | ||
| + | |||
| + | gel extraction | ||
OHS vector Conc. 38ng/ul | OHS vector Conc. 38ng/ul | ||
pSB1C3 vector Conc. 55ng/ul | pSB1C3 vector Conc. 55ng/ul | ||
| - | --[[User:Zora|Zora]] 01:28, 2 September 2011 (CDT) | + | --[[User:Zora|Zora]] 01:28, 2 September 2011 (CDT) |
Revision as of 07:54, 2 September 2011
draw line pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate --Zora 02:54, 2 September 2011 (CDT)
digest pSB1C3 5ug digest with NEB EcoRI&SpeI
pet15a - OHS parts
new plasmid
gel analysis OmpA-HIV cleavage site-Sh3 digested done by ZLY pSB1C3 digestedgel extraction OHS vector Conc. 38ng/ul pSB1C3 vector Conc. 55ng/ul --Zora 01:28, 2 September 2011 (CDT)
