"
Team:Caltech/Week 12
From 2011.igem.org
| Line 21: | Line 21: | ||
Overnights of DDT-pET, WT-F87A, and lacZ colonies for sequencing, BioBrick submission, spectrophotometer experiments, etc.<br/> | Overnights of DDT-pET, WT-F87A, and lacZ colonies for sequencing, BioBrick submission, spectrophotometer experiments, etc.<br/> | ||
Make M9 media for suspending X-gal for biofilm column experiments<br/> | Make M9 media for suspending X-gal for biofilm column experiments<br/> | ||
| + | Obtain pumps, tubing, and clear columns for biofilm-X-gal experiment<br/> | ||
===Results=== | ===Results=== | ||
HPLC results revealed too much noise; chemicals at too low concentration<br/> | HPLC results revealed too much noise; chemicals at too low concentration<br/> | ||
Revision as of 00:07, 2 September 2011
|
Project |
August 28Made R0010-MG1655 and J23119-MG1655 plates and overnights ResultsJ23119-MG1655 grew, but the R0010-MG1655 did not August 29R0010 overnight culture did not grow ResultsJ23119 culture was too thick and not in exponential growth phase; redo August 30HPLC of yesterday's p450 reaction and purification ResultsHPLC results revealed too much noise; chemicals at too low concentration August 31Miniprep and sequencing of DDT-pET with T7 promoter and terminator, as well as WT-F87A using internal primers ResultsR0010 glycerol stocks are not growing overnights September 1Sequencing of DDT-pET revealed correct results ResultsHPLC did not yield expected results
|
