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Team:Paris Liliane Bettencourt/Notebook/2011/09/01/
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==Danyel== | ==Danyel== | ||
| + | ===GFP amber=== | ||
| + | Quick change mutation was launched last night of: GFPmut3b (E0040) and GFP + ter with the same primers. | ||
| + | Today, PCR products were digested with DpnI FastDigest enzymes and run on gel. | ||
| + | [[File:GFP QM Gel.jpg]] | ||
Revision as of 19:10, 1 September 2011
Contents |
Cyrille
Troubleshoting K606003
The glycerol was stricked on a plate. The region where the plasmids are too concentrated loose the plasmid.
Miniprep of pHM3 from glycerol
Two miniprep of pHM3 where done.
Cloning of YFP-TetR
The strain K1 (containing LacO) was open in SP. The digestion was PCR Purified.
The pHM3 was digested in EP and gel purified, but it didn't gave any yield.
There where very few of YFP-TetR left. The digested product was not visible on the gel.
Launch again YFP-TETR culture
The glycerol YFP TetR was launched again in 3 tube for miniprep tomorow
Comptetent cell debbuging and preparation
There where ampicylin contamination in comptetent cells. Turbocells where stricken on a plate for competent cell preparation tomorow.
Danyel
GFP amber
Quick change mutation was launched last night of: GFPmut3b (E0040) and GFP + ter with the same primers.
Today, PCR products were digested with DpnI FastDigest enzymes and run on gel.
