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Team:Caltech/Week 12
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| + | ==August 28== | ||
| + | Made R0010-MG1655 and J23119-MG1655 plates and overnights | ||
| + | Continued growing biofilm in flask with glass beads; added fresh amphicillin | ||
| + | Mixed more X-gal and IPTG solution at 20mg/mL and .1M concentrations respectively | ||
| + | Attempted to induce R0010 overnights with IPTG to cleave X-gal; J23119 to cleave X-gal without inducing | ||
| + | ===Results=== | ||
| + | J23119-MG1655 grew, but the R0010-MG1655 did not | ||
| + | R0010 did not cleave X-gal even with a high concentration of both IPTG and X-gal | ||
| + | J23119 cultures turned blue with cleaved X-gal after about 4 minutes | ||
| + | |||
| + | ==August 29== | ||
| + | R0010 overnight culture did not grow | ||
| + | Redo p450 experiments in MeOH in order to send to electrospray | ||
| + | Attempt to measure J23119-X-gal reaction with spectrophotometer | ||
| + | ===Results=== | ||
| + | J23119 culture was too thick and not in exponential growth phase; redo | ||
| + | |||
| + | ==August 30== | ||
| + | HPLC of yesterday's p450 reaction and purification | ||
| + | Overnights of DDT-pET, WT-F87A, and lacZ colonies for sequencing, BioBrick submission, spectrophotometer experiments, etc. | ||
| + | Make M9 media for suspending X-gal for biofilm column experiments | ||
| + | ===Results=== | ||
| + | HPLC results revealed too much noise; chemicals at too low of concentration | ||
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Revision as of 02:21, 1 September 2011
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Project |
August 28Made R0010-MG1655 and J23119-MG1655 plates and overnights Continued growing biofilm in flask with glass beads; added fresh amphicillin Mixed more X-gal and IPTG solution at 20mg/mL and .1M concentrations respectively Attempted to induce R0010 overnights with IPTG to cleave X-gal; J23119 to cleave X-gal without inducing ResultsJ23119-MG1655 grew, but the R0010-MG1655 did not R0010 did not cleave X-gal even with a high concentration of both IPTG and X-gal J23119 cultures turned blue with cleaved X-gal after about 4 minutes August 29R0010 overnight culture did not grow Redo p450 experiments in MeOH in order to send to electrospray Attempt to measure J23119-X-gal reaction with spectrophotometer ResultsJ23119 culture was too thick and not in exponential growth phase; redo August 30HPLC of yesterday's p450 reaction and purification Overnights of DDT-pET, WT-F87A, and lacZ colonies for sequencing, BioBrick submission, spectrophotometer experiments, etc. Make M9 media for suspending X-gal for biofilm column experiments ResultsHPLC results revealed too much noise; chemicals at too low of concentration
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