Team:Freiburg/Notebook/26 August

From 2011.igem.org

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(→blue light receptor)
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===NAME OF YOUR EXPERIMENT===
===NAME OF YOUR EXPERIMENT===
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Transformation
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Name: Sophie
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Date: 26.08.11
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Continue from                                    Date: 26.08.11        Name: Sophie               
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Experiment Ligation   
 +
Project Name: Blue light receptor
-
'''Investigators:NAME'''
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Procedure
 +
1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
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2. thaw cells on ice 20 minutes
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3. pipette 50 μl cells and 2 μl DNA into eppi still on ice!
 +
4. Incubate for 30 minutes on ice
 +
5. Heat at 42°C for 60 sec
 +
6. Incubate on ice for 5 minutes
 +
7. Add 200 μl LB Broth
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8. Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
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9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
 +
Documentation:
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Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
 +
Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.
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Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3
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stored in incubator on Amp plates
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vector: psb1A3
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inserts: Not/ nOt/ noT + LOV-3A-PCR
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 +
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'''Investigators:NAME'''
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 12:44, 26 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Meeting

attendants:
Time:

green light receptor

already done:


To-do:

blue light receptor

already done:


To-do:

red light receptor

already done:


To-do:

Lysis cassette

already done:


To-do:


Precipitator

already done:


To-do:

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Transformation Name: Sophie Date: 26.08.11 Continue from Date: 26.08.11 Name: Sophie Experiment Ligation Project Name: Blue light receptor

Procedure

1. take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells) 2. thaw cells on ice 20 minutes 3. pipette 50 μl cells and 2 μl DNA into eppi still on ice! 4. Incubate for 30 minutes on ice 5. Heat at 42°C for 60 sec 6. Incubate on ice for 5 minutes 7. Add 200 μl LB Broth 8. Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!) 9. Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance


Documentation: Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc. Why? The last cloning was done with a NOT-PCR-Product which might make problems because it does not have enough bases after the restriciton sites.

Name of the samples: Not 1:1, Not 1:3, nOt 1:1 nOt 1:3, noT 1:1, noT 1: 3

stored in incubator on Amp plates

vector: psb1A3 inserts: Not/ nOt/ noT + LOV-3A-PCR


Investigators:NAME

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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