Team:Caltech/Week 11

From 2011.igem.org

(Difference between revisions)
Line 15: Line 15:
* 16 LB-chlor plates  
* 16 LB-chlor plates  
* 13 LB plates  
* 13 LB plates  
 +
==August 24==
 +
==August 25==
 +
===Results===
 +
<table border="1">
 +
<tr>
 +
<th>Name</th>
 +
<th>Number of colonies</th>
 +
</tr>
 +
<tr>
 +
<td>p450 biobricked in pSB1C3</td>
 +
<td>~3400</td>
 +
</tr>
 +
<tr>
 +
<td>DDT gene biobrick now with stop codons in pSB1C3</td>
 +
<td>~6400</td>
 +
</tr>
 +
<tr>
 +
<td>pSB1C3 ligation negative control</td>
 +
<td>0</td>
 +
</tr>
 +
<tr>
 +
<td>DDT gene inserted into pET11-a</td>
 +
<td>~6400</td>
 +
</tr>
 +
<tr>
 +
<td>pET11-a ligation negative control</td>
 +
<td>27</td>
 +
</tr>
 +
<tr>
 +
<td>pUC 19 in DH5a electrocompetent cells</td>
 +
<td>976</td>
 +
</tr>
 +
<tr>
 +
<td>DDT gene PIPE</td>
 +
<td>0</td>
 +
</tr>
 +
<tr>
 +
<td>VPIPE negative control</td>
 +
<td>0</td>
 +
</tr>
 +
<tr>
 +
<tr>
 +
<td>pNT002 + in commercial XL-10 gold</td>
 +
<td>54</td>
 +
</tr>
 +
<tr>
 +
<td>pNT002 - in commercial XL-10 gold</td>
 +
<td>28</td>
 +
</tr>
 +
<tr>
 +
</table>
}}
}}

Revision as of 22:25, 25 August 2011


Caltech iGEM 2011



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August 22

Send BPA and degraded products to HPLC for analysis
Send 16s for sequencing using FWD and REV.

Results

Plated 16s and WT-F87A cultures show many colonies
Mix 10% glycerol solution and place in 4&degC fridge for tomorrow's electrocompetence method
Overnight shake culture of MG-1655 experimental E. coli strain

August 23

Make experimental MG-1655 strain electrocompetent
Made 53 plates today!

  • 24 LB-amp plates
  • 16 LB-chlor plates
  • 13 LB plates

August 24

August 25

Results

Name Number of colonies
p450 biobricked in pSB1C3 ~3400
DDT gene biobrick now with stop codons in pSB1C3 ~6400
pSB1C3 ligation negative control 0
DDT gene inserted into pET11-a ~6400
pET11-a ligation negative control 27
pUC 19 in DH5a electrocompetent cells 976
DDT gene PIPE 0
VPIPE negative control 0
pNT002 + in commercial XL-10 gold 54
pNT002 - in commercial XL-10 gold 28


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