Team:Freiburg/Notebook/22 August

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PCR product was loaded onto a gel. Expected lane: 900bp
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PCR product was loaded onto a gel. Expected lane: 900bp<br/>
PCR worked and we got Not-Gate with NEHI and PstI.
PCR worked and we got Not-Gate with NEHI and PstI.

Revision as of 09:17, 24 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Sequencing

Investigators: Sandra

The sequencing showed that the 3A-assembly did not work. We will send the LovTap PCR product to get sequenced. May be the PCR did not work.

PCR

Investigators: Sandra

PCR of Not-Gate.

P 74:

  • actgcagcggccgctgctagca

P 24:

  • tatgaattcgcggccgcttctag


PCR product was loaded onto a gel. Expected lane: 900bp
PCR worked and we got Not-Gate with NEHI and PstI.

Cloning

Investigators: Sophie

new 3A assembly with Sandra's NOT-gate-PCR-product, our LOV-Tap-pcr-product (both mutated with NEH1 restriction sites) and an amp-vector
The digested samples were loaded to a gel. They showed clear bands in the right size. Only the band of the vector was not intense enough, so the vector has to be digested again.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Transformation

Investigators: Rüdiger, Sandra

Transformation of 61,62 and 63.
Vector:pSB1C3

Incubation over night.


Cloning for GFP-pbd with IPTG-inucible promoter

Investigator: Sophie
as cloning from 18.08.11 did not work I try it again with longer digestion times.

                       

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