Team:Baltimore/Hardware

From 2011.igem.org

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(Current Hardware Stuff)
(Current Hardware Stuff)
 
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*Remove block entirely and construct PCR from heated baths
*Remove block entirely and construct PCR from heated baths
*A mixture of a few of these (simpler block with baths rather then tube shaped holes)
*A mixture of a few of these (simpler block with baths rather then tube shaped holes)
 +
 +
<br>Heating Rate: .7 to 1.1 C/S
 +
<br>Cooling Rate: 2.0 C/S
'''PCR Design:''' <br>
'''PCR Design:''' <br>
[[File:Bmore_PCR_Design.png]]
[[File:Bmore_PCR_Design.png]]
<br>
<br>
-
 
+
<br>
-
''Cheap PCR (maybe?)'''<br>
+
<br>
 +
'''Cheap PCR (maybe?)'''<br>
[[File:Bmore_CheapPCR_Design.png]] <br>
[[File:Bmore_CheapPCR_Design.png]] <br>
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[[File:Bmore_CheapBlock_Design.png]] <br>
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'''Software/Interfacing stuff'''
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<br><br>
 +
For the PCR interface software we need: <br>
 +
*A menu bar with save, load, PCR settings, about, comm settings, connect, and disconnect
 +
*A list box that allows us to add time/level points.
 +
*A set of textboxes that we input new times/levels in (with a button)
 +
*A picture box depicting the heating profile we are setting
 +
*A set of textboxes/labels that indicate the current state of the PCR.
 +
*A set of buttons, execute the current sequence, reset the sequence, and stop/pause the sequence.

Latest revision as of 19:17, 22 August 2011

Home Team Project Parts Submitted to the Registry Modeling Notebook Solutions Safety Attributions Hardware Material Safety Data Sheets [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry]



Current Hardware Stuff

Tube shims:
PCRShim.png
Tube shims for centerfuge are finished. They will be uploaded to shapeways if anyone wants to get 'em.
Should be cheap. Will modify to cheapen.

PCR:
PCRBlock1.png
Current PCR block. Quotes for it's manufacture are woefully high. Looking to decrease this cost. Prices:

  • $950 (!!!) to have it 3D printed.
  • ~$300 to $400 for a custom machining job.
  •  ??? Other methods of manufacture (sand casting, investment casting, ect...)?

Ways to possibly lower the cost:

  • A Heat conductive polymer
  • Some kind of putty (?? No idea what this was ??)
  • Ceramic block
  • Decrease size of block
  • Remove hard to machine features
  • Remove block entirely and construct PCR from heated baths
  • A mixture of a few of these (simpler block with baths rather then tube shaped holes)


Heating Rate: .7 to 1.1 C/S
Cooling Rate: 2.0 C/S

PCR Design:
Bmore PCR Design.png


Cheap PCR (maybe?)
Bmore CheapPCR Design.png
Bmore CheapBlock Design.png

Software/Interfacing stuff

For the PCR interface software we need:

  • A menu bar with save, load, PCR settings, about, comm settings, connect, and disconnect
  • A list box that allows us to add time/level points.
  • A set of textboxes that we input new times/levels in (with a button)
  • A picture box depicting the heating profile we are setting
  • A set of textboxes/labels that indicate the current state of the PCR.
  • A set of buttons, execute the current sequence, reset the sequence, and stop/pause the sequence.