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Team:KULeuven/Workshop
From 2011.igem.org
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<b>How to isolate DNA?</b><br> | <b>How to isolate DNA?</b><br> | ||
| - | + | <ul class="sum_number"> | |
| - | + | <li>Cut a tomato into pieces and put it into a bowl.</li> | |
| + | <li>Make a solution of ... (in another bowl) | ||
| + | <ul class="sum_dot"> | ||
| + | <li>5 ml Detergent</li> | ||
| + | <li>1 teaspoon of salt</li> | ||
| + | <li>45 ml water</li></ul> | ||
| + | <li>Add the solution to the pieces of tomato.</li> | ||
| + | <li>Use the whisk to mix the solution with the pieces of tomato. | ||
| + | <li>Put the filter in the funnel and pour the tomato - solution mix in the funnel. The filtrate contains DNA.</li> | ||
| + | <li>To make the DNA visible: | ||
| + | <ul class="sum_dot"> | ||
| + | <li>Pipet 0,5 ml of the filtrate in an Eppendorf tube</li> | ||
| + | <li>Add 1 ml of Ethanol</li> | ||
| + | <li>Close the Eppendorf tube</li> | ||
| + | <li>Turn the Eppendorf tube with a gentle movement up and down.</li></ul> | ||
| + | <li>The red color pellet (looks like snot) is DNA.</li> | ||
| + | </ul> | ||
</div> | </div> | ||
Revision as of 11:25, 22 August 2011
WORKSHOP
The team has organized a workshop for children aged 9-12 years old on the 17th of August in Oud-Heverlee. They have explained synthetic biology in a playful way and afterwards the children drew their own visions on synthetic biology and what they would like to create with synthetic biology. A mini-documentary is being made and will be posted on our wiki shortly. The workshop was also filmed by ROB TV and this report was recently broadcasted on their network (http://robtv.be/nieuws/oud-heverlee/tienjarigen-ontdekken-dna).Protocol to isolate the DNA of a tomato
What do we need?- Tomato
- Salt
- Water
- Detergent
- Ethanol (or whiskey/vodka)
- 3 bowls
- Knife (plastic for the kids)
- A whisk
- Microliter pipet and pipet tips
- Eppendorf tubes
- Filters (coffee filters)
- Funnel
How to isolate DNA?
- Cut a tomato into pieces and put it into a bowl.
- Make a solution of ... (in another bowl)
- 5 ml Detergent
- 1 teaspoon of salt
- 45 ml water
- Add the solution to the pieces of tomato.
- Use the whisk to mix the solution with the pieces of tomato.
- Put the filter in the funnel and pour the tomato - solution mix in the funnel. The filtrate contains DNA.
- To make the DNA visible:
- Pipet 0,5 ml of the filtrate in an Eppendorf tube
- Add 1 ml of Ethanol
- Close the Eppendorf tube
- Turn the Eppendorf tube with a gentle movement up and down.
- The red color pellet (looks like snot) is DNA.
