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Team:Caltech/Week 10
From 2011.igem.org
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CPCR of Gibson pNT002 and pNT003<br/> | CPCR of Gibson pNT002 and pNT003<br/> | ||
Miniprep J23119 and I732019 to construct LacZ producer<br/> | Miniprep J23119 and I732019 to construct LacZ producer<br/> | ||
| - | GCMS of bisphenol A dissolved in methanol | + | GCMS of bisphenol A dissolved in methanol <br/> |
| + | Gibson 16s sequencing DNA and vector together<br/> | ||
===Results=== | ===Results=== | ||
CPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked.<br/> | CPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked.<br/> | ||
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Electrospray bisphenol A dissolved in methanol<br/> | Electrospray bisphenol A dissolved in methanol<br/> | ||
Attempt to standard assemble constitutively expressed LacZ and transform into XL10 gold. However, there was no good way to select out the lacZ insert (I732019) from the backbone it was cut out of, as they are the same length.<br/> | Attempt to standard assemble constitutively expressed LacZ and transform into XL10 gold. However, there was no good way to select out the lacZ insert (I732019) from the backbone it was cut out of, as they are the same length.<br/> | ||
| - | + | Nanodrop 16s solutions <br/> | |
===Results=== | ===Results=== | ||
Electrospray results show correct mass spectrometry peaks<br/> | Electrospray results show correct mass spectrometry peaks<br/> | ||
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==August 17== | ==August 17== | ||
Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces<br/> | Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces<br/> | ||
Revision as of 18:44, 18 August 2011
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Project |
August 15CPCR of Gibson pNT002 and pNT003 ResultsCPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked. August 16Electrospray bisphenol A dissolved in methanol ResultsElectrospray results show correct mass spectrometry peaks August 17Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces ResultsThe lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.
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