Team:Glasgow/Product 1 Generating Device

From 2011.igem.org

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<h1>Product One Generating Device Construct</h1>
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<h1>Product Three Generating Device Construct</h1>
<p>Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.</p>
<p>Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.</p>
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   <td id="leftcolumn" width="122" align="center" valign="top"><p><b>Blue Light Dependant Promoter</b>
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   <td id="leftcolumn" width="122" align="center" valign="top"><p><b>Green Light Dependant Promoter</b>
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       <br /><a href="http://partsregistry.org/Part:BBa_K238013">BBa_K238013</a></p>
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       <br /><a href="http://partsregistry.org/Part:BBa_K225000">BBa_K225000</a></p>
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       <p>Weak blue light dependant promoter. Strength is considered reletive to I20260
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       <p>Red light dependant promoter developed by the 2004 UT Austin iGEM team.  
   <td id="leftcolumn" width="195" align="center" valign="top"><p><b>Strong Ribosome Binding Site</b>
   <td id="leftcolumn" width="195" align="center" valign="top"><p><b>Strong Ribosome Binding Site</b>
       <br /><br /><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></p>
       <br /><br /><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></p>
       <p>Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034</p></td>
       <p>Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034</p></td>
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   <td id="leftcolumn" width="165" align="center" valign="top"><p><b>Colanic Acid</b>
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   <td id="leftcolumn" width="165" align="center" valign="top"><p><b>Beta-carotene</b>
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       <br /><br /><a href="http://partsregistry.org/Part:BBa_K274100">BBa_K274100</a></p>
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       <br /><br /><a href="http://partsregistry.org/Part:BBa_K274200">BBa_K274200</a></p>
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       <p>Biobrick designed by the 2009 Cambridge team. Converts FPP to lycopene. Lycopene is a red coloured carotinoid.</p>
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       <p>Biobrick designed by the 2009 Cambridge team. Converts FPP to beta-carotene. Beta-carotene is an orange coloured carotinoid.</p>
   <td id="leftcolumn" width="150" align="center" valign="top"><p><b>Double Terminator</b>
   <td id="leftcolumn" width="150" align="center" valign="top"><p><b>Double Terminator</b>
       <br /><br /><a href="http://partsregistry.org/Part:BBa_B1006">BBa_B1006</a></p>
       <br /><br /><a href="http://partsregistry.org/Part:BBa_B1006">BBa_B1006</a></p>

Latest revision as of 09:59, 18 August 2011


Product Three Generating Device Construct

Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.

Green Light Dependant Promoter
BBa_K225000

Red light dependant promoter developed by the 2004 UT Austin iGEM team.

Strong Ribosome Binding Site

BBa_B0030

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034

Beta-carotene

BBa_K274200

Biobrick designed by the 2009 Cambridge team. Converts FPP to beta-carotene. Beta-carotene is an orange coloured carotinoid.

Double Terminator

BBa_B1006

Artificial double terminator with 8bp stem and 6 nucleotide loop