Team:Glasgow/Product 1 Generating Device

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Team:Glasgow/Header}} <html> <head> <style> div.construct { position:relative; width:756px; padding:10px; left:62px; border: 1px solid #000000; } </style> </head> <body> <br />...")
Line 15: Line 15:
<body>
<body>
<br />
<br />
-
<h1>Product One Selection Device Construct</h1>
+
<h1>Product One Generating Device Construct</h1>
<p>Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.</p>
<p>Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.</p>
</p>
</p>

Revision as of 09:34, 18 August 2011


Product One Generating Device Construct

Construct that is regulated by a blue light inducible promoter. Allows for selection of a any product which needs specific controlled expression either temporally or spatially. For proof of concept coloured carotinoids will be used, however in practice these products could be enzymes in a metabolic pathway that can be selectively induced to create different end products from the same precursor molecule.

Blue Light Dependant Promoter
BBa_K238013

Weak blue light dependant promoter. Strength is considered reletive to I20260

Strong Ribosome Binding Site

BBa_B0030

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034

Colanic Acid

BBa_K274100

Biobrick designed by the 2009 Cambridge team. Converts FPP to lycopene. Lycopene is a red coloured carotinoid.

Double Terminator

BBa_B1006

Artificial double terminator with 8bp stem and 6 nucleotide loop