Team:Freiburg/Notebook/17 August

From 2011.igem.org

(Difference between revisions)
(→Commons)
(→NAME OF YOUR EXPERIMENT)
Line 90: Line 90:
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Miniprep===
-
'''Investigators:NAME'''
+
'''Investigators: Sandra'''
 +
Miniprep of LovTAP-NotGate-T3:
 +
*15ng/μl
 +
stored in undigested miniprep box II
 +
 +
===Testdigest===
 +
 +
'''Investigators: Sandra'''
 +
 +
Testdigest of Miniprep product
 +
 +
Enzymes:
 +
*EcoRI
 +
*PstI
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 13:40, 17 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Commons

PCR


Name: Sophie


Date: 17.08.11
Continue from Experiment (Date)

(Name): Commons

Project Name: tet-vector seems to make problems -> new PCR with original psB1T3-DNA

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20 Name
10µl 5x Phusion Buffer of Primer
2.5µl Primer fw SB-prep-3P-1
2.5µl Primer dw SB-prep-2Ea
1µl dNTPs of Template DNA
1µl DNA-Template

PSB 1 T3

0.5 µl Phusion (add in the end)

What program do you use?

  1. 2Min 94°C
  2. 30s 94°C
  3. 30s 55°C
  4. 3min 72°C
  5. 10min 72°C

step 2,3 and 4 in 35 cycles


To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.


How did you label the PCR-Product, where is it stored and what do you do next?

Labeled PSB 1 T3 stored in -20°C in last drawer

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

Miniprep

Investigators: Sandra

Miniprep of LovTAP-NotGate-T3:

  • 15ng/μl

stored in undigested miniprep box II


Testdigest

Investigators: Sandra

Testdigest of Miniprep product

Enzymes:

  • EcoRI
  • PstI

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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