Team:Tianjin/Project

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<!--- The Mission, Experiments --->
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!align="center"|[[Team:Tianjin|Home]]
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!align="center"|[[Team:Tianjin/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Tianjin Official Team Profile]
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!align="center"|[[Team:Tianjin/Project|Project]]
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!align="center"|[[Team:Tianjin/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Tianjin/Modeling|Modeling]]
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== '''Project Abstract: Y’s multi-guard''' ==
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Decades of years have passed since the concept of "Synthetic Biology" being introduced, it is now essential to view cells as true ‘programmable’ entities, and develop effective strategies for assembling devices and modules into intricate, customizable larger scale systems rather than creation and perfection of genetic devices and small modules based on them. Meanwhile, regulations on gene expression have evolved from transcription, to translation, and finally signal transduction level.  
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This year we are aimed at increasing the tolerance of Saccharomyces cerevisiae to inhibitors in lignocellulosic hydrolysates, such as weak acids, furans and phenolic compounds formed during pretreatment and hydrolysis. The core of our project is directional modification of TOR (The target of rapamycin), a highly conserved Ser/Thr protein kinase which is the central component of a major signaling transduction network that controls cell growth in diverse eukaryotic organisms, ranging from yeast to man. Former research such as transcriptomics, proteomic and metabonomics results show that inhibitors, especially acetate, would a series of phenotypic and intracellular changes induced by TOR complex, including the entry of some transcription factors to the nucleus, some cell cycle arrest and entry into G0, general downregulation of protein synthesis, accumulation of the reserve carbohydrate glycogen, upregulation of stress response genes, autophagy and alterations in nitrogen and carbon metabolism. Thus we are seeking for ways to mutate the TOR to make it ignorant of the inhibiting signal to maintain normal physiological activity and keep the overall signaling pathway on the right direction.
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Noticeable work has been done on mTORC1 (mammalian TOR complex 1) that modulation in certain amino acid sites could promote the activity in response to adverse nutrient status and inhibitors, while same work has not been done on Saccharomyces cerevisiae. However, the high homogeneity between TOR and mammalian TOR makes it possible to transplant the modulation into Saccharomyces cerevisiae. We separated TOR1, the key part of TORC1, into three parts and picked up 4 amino acid sites. Gibson Assembly and overlap PCR have been used for the introduction of site-directed mutagenesis.
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Besides the mutation of TOR, a complete genetic circuit is needed to achieve the function. We tested different transcriptional factors and promoters which used to be regulated by the unmutated TOR and found out the optimum combination.
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Finally, all the modules will be integrated into a YAC (Yeast Artificial Chromosome). We hope to make it become the 17th chromosome of the yeast (normally 16). With this additional chromosome, the yeast will gain stable growth, high yield and productivity of ethanol and an extension in life span regardless of the existence of inhibitors in lignocellulosic hydrolysates. Furthermore, we also want to apply this integrated YAC with different functions and into others kinds of organism. That is to say, with this universal and standard module, we can put it into any organism needs to be remodeled, just like an USB.
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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        <ul>
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                <a href="#"><img src="https://2011.igem.org/File:TJU-Project-head.png"/></a>
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                <li class="subtitle"><a href="#t1">1 xxxx</a>
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                    <ul>
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                        <li><a href="#t11">1.1 xxx</a></li>
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                            <li><a href="#t12">1.2 xxxx</a></li>
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                        </ul>
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                    <li class="subtitle"><a href="#t2">2 xxxx</a>
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                        <li><a href="#t21">2.1 xxx</a></li>
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                    <li class="subtitle"><a href="#t3">3 xxxx</a>
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                    <ul>
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                        <li><a href="#t31">3.1 xxx</a></li>
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                        </ul>
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                </li>
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                    <li class="subtitle"><a href="#t4">4 xxxx</a></li>
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            <h1 id="t1">1 xxxx</h1>
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<h2 id="t11">1.1 xxx</h2>
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            <p>&nbsp;&nbsp;
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                On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.
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                </p>
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        <p>&nbsp;&nbsp;
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                During the lecture, Professor Chen generally reviewed the course of struggle and brilliant achievements that the CPC has made since its founding in 1921. She also illustrated the concept of socialism with Chinese characteristics in great detail. In the two-hour lecture, Prof. Chen integrated abundant historical facts into the theoretical discussion and fully displayed the spirit and vitality of Marxism.
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        <h2 id="t12">1.2 xxxx</h2>
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                <p>&nbsp;&nbsp;
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                In innovative talent cultivation, the School strives to reform course system and update course content catering to social needs and industry development. It also introduces excellent original foreign textbooks, and increases the percentage of bilingual programs in the whole curriculum. Besides, it steps up the communication and cooperation with famous universities and enterprises in the world to jointly train talents specialized in software engineering and animation who can meet the needs of the society. All these efforts are dedicated to increase the impact of Tianjin University on the Bohai Rim area, to enhance the strength of the School and to make contribution to the social and economic development of Binhai New Area.
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                </p>
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        <h1 id="t2">2 xxxx</h1>
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        <h2 id="t21">2.1 xxx</h2>
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            <p>&nbsp;&nbsp;
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                On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.
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                </p>
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        <h1 id="t3">3 xxxx</h1>
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        <h2 id="t31">3.1 xxx</h2>
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            <p>&nbsp;&nbsp;
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                On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.
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Revision as of 05:22, 17 August 2011

Team:Tianjin/Project

1 xxxx

1.1 xxx

   On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.

   During the lecture, Professor Chen generally reviewed the course of struggle and brilliant achievements that the CPC has made since its founding in 1921. She also illustrated the concept of socialism with Chinese characteristics in great detail. In the two-hour lecture, Prof. Chen integrated abundant historical facts into the theoretical discussion and fully displayed the spirit and vitality of Marxism.

1.2 xxxx

   In innovative talent cultivation, the School strives to reform course system and update course content catering to social needs and industry development. It also introduces excellent original foreign textbooks, and increases the percentage of bilingual programs in the whole curriculum. Besides, it steps up the communication and cooperation with famous universities and enterprises in the world to jointly train talents specialized in software engineering and animation who can meet the needs of the society. All these efforts are dedicated to increase the impact of Tianjin University on the Bohai Rim area, to enhance the strength of the School and to make contribution to the social and economic development of Binhai New Area.

2 xxxx

2.1 xxx

   On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.

3 xxxx

3.1 xxx

   On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.On May 6, 2011, School of Electronic and Information Engineering, School of Computer Science and Technology and School of Computer Software jointly held a lecture on the history of CPC, which also launched the "Excel in the Performance" activities co-hosted by information technology and science discipline group. Chen Xuewei, a famous specialist on the theory of CPC history, and professor of Party School of the Central Committee of CPC was invited by the three schools to make a lecture on the topic of "Great Rejuvenation of CPC and the Chinese Nation". Several senior officials from the University and the hosting schools attended the ceremony, and about 420 students took part in the activity.

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