Team:Colombia/Notebook

From 2011.igem.org

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(→iGemColombia Notebook)
(→iGemColombia Notebook)
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* Colonies from bricks 1, 3, 4 and 5 were stinged.
* Colonies from bricks 1, 3, 4 and 5 were stinged.
<blockquote class="Task">
<blockquote class="Task">
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: Task:  
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: ''Task:''
: To make LB medium (15x25mL)
: To make LB medium (15x25mL)
</blockquote>
</blockquote>
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* Liquid LB was prepared (400mL).
* Liquid LB was prepared (400mL).
<blockquote class="Task">
<blockquote class="Task">
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: Task:  
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: ''Task:''
: To add ampiciline and tetracicline to the boxes.
: To add ampiciline and tetracicline to the boxes.
: Claim the liquid LB
: Claim the liquid LB
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* Direct inoculation x 2 and C(-) MgCl2.
* Direct inoculation x 2 and C(-) MgCl2.
<blockquote class="Task">   
<blockquote class="Task">   
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: Task:  
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: ''Task:''
: Print electroporation protocol.
: Print electroporation protocol.
: Ask Juan D. Olarte about the inoculation in coffee.
: Ask Juan D. Olarte about the inoculation in coffee.
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* Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
* Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
<blockquote class="Task">
<blockquote class="Task">
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: Task:
+
: ''Task:''
: To finish the minipreps from the addition of RNAsa.
: To finish the minipreps from the addition of RNAsa.
: Check the E. Coli growth in coffee.
: Check the E. Coli growth in coffee.
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* Transformation protocol in chemical cells.
* Transformation protocol in chemical cells.
<blockquote class="Task">
<blockquote class="Task">
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: Task:
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: ''Task:''
: Prepare 250 mL of SOC medium
: Prepare 250 mL of SOC medium
: Print the Transformation protocol for chemical cells.
: Print the Transformation protocol for chemical cells.
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* E. Coli growth results.
* E. Coli growth results.
<blockquote class="Task">
<blockquote class="Task">
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: Task:
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: ''Task:''
: Print the Transformation protocol for chemical cells.
: Print the Transformation protocol for chemical cells.
: Competent cells for clons 2 and 5.
: Competent cells for clons 2 and 5.
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* Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
* Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
<blockquote class="Task">
<blockquote class="Task">
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: Task:  
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: ''Task:''
: Print the Transformation protocol for chemical cells.
: Print the Transformation protocol for chemical cells.
: Preserve brick 3.
: Preserve brick 3.
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* Bricks 7, 9 and 10 were contaminated.
* Bricks 7, 9 and 10 were contaminated.
<blockquote class="Task">
<blockquote class="Task">
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: Task:
+
: ''Task:''
: Print the chemical cells protocol.
: Print the chemical cells protocol.
: Confirm all biobricks (Digestion).
: Confirm all biobricks (Digestion).

Revision as of 05:05, 16 August 2011

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Contents

iGemColombia Notebook

Here you can find our daily work in the Lab!

June

June 30:

  • Biobricks 1, 2, 3, 4 and 5 were resuspended
  • Miniprep Solutions (I, II and III) were prepared.

July

July 1

  • Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

  • Biobrick 2 presented no colonies.
  • Colonies from bricks 1, 3, 4 and 5 were stinged.
Task:
To make LB medium (15x25mL)

July 6

  • Biobricks 1, 3 and 4 were twice plated.
  • Brick 5 didn't grow up.
  • We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
  • LB medium was prepared (15x25mL).
  • Liquid LB was prepared (400mL).
Task:
To add ampiciline and tetracicline to the boxes.
Claim the liquid LB
Scrape ....
Sting biobricks 2 and 5 (no clons)
Pick up the tubes with Merceditas

July 7

  • Clons 2 and 5 didn't work out.
  • Clons 1, 3 and 4 were planted in LB liquid.
  • E. Coli inoculation in Coffee
  • Kanamicine resistence plasmid: 0.2 optic density.
  • Direct inoculation x 2 and C(-) MgCl2.
Task:
Print electroporation protocol.
Ask Juan D. Olarte about the inoculation in coffee.
Minipreps for confirmation of 1, 3 and 4.
Competent cells for clons 2 and 5.

July 8

  • Minipreps for 1, 3 and 4 were made.
  • Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
Task:
To finish the minipreps from the addition of RNAsa.
Check the E. Coli growth in coffee.

July 11

  • Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
  • Transformation protocol in chemical cells.
Task:
Prepare 250 mL of SOC medium
Print the Transformation protocol for chemical cells.

July 12

  • Strains 1 and 4 have been conserved.
  • LB liquid culture was prepared again for brick 3.
  • E. Coli growth results.
Task:
Print the Transformation protocol for chemical cells.
Competent cells for clons 2 and 5.
Preserve brick 3.
Confirm bricks 1, 3 and 4 (Digestion)
Resuspend all Biobricks.
Check the E. Coli growth in coffee.

July 14

  • E. Coli didn't grow up on the sheets.
  • Chemical competent cells were made (DH5α).
  • Brick 3 was left to grow in liquid medium.
  • Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
Task:
Print the Transformation protocol for chemical cells.
Preserve brick 3.
Confirm all biobricks (Digestion)
Sting the transformed bricks.
Add antibiotic to the mediums made today.

July 15

  • Brick 3 was preserved in Revco.
  • Bricks 2, 5 and 8 were stinged.
  • 25 LB+Kan boxes x 25 mL.
  • No colonies in brick 6.
  • Bricks 7, 9 and 10 were contaminated.
Task:
Print the chemical cells protocol.
Confirm all biobricks (Digestion).

August

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