Team:Colombia/Notebook

From 2011.igem.org

(Difference between revisions)
(→iGemColombia Notebook)
(→iGemColombia Notebook)
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* Liquid LB was prepared (400mL).
* Liquid LB was prepared (400mL).
<blockquote class="Task">
<blockquote class="Task">
-
:     Task:  
+
: Task:  
-
:     To add ampiciline and tetracicline to the boxes.
+
: To add ampiciline and tetracicline to the boxes.
-
:     Claim the liquid LB
+
: Claim the liquid LB
-
:     Scrape ....
+
: Scrape ....
-
:     Sting biobricks 2 and 5 (no clons)
+
: Sting biobricks 2 and 5 (no clons)
-
:     Pick up the tubes with Merceditas
+
: Pick up the tubes with Merceditas
</blockquote>
</blockquote>
===July 7===
===July 7===
Line 36: Line 36:
* Kanamicine resistence plasmid: 0.2 optic density.
* Kanamicine resistence plasmid: 0.2 optic density.
* Direct inoculation x 2 and C(-) MgCl2.
* Direct inoculation x 2 and C(-) MgCl2.
-
      Task:  
+
<blockquote class="Task"> 
-
      Print electroporation protocol.
+
: Task:  
-
      Ask Juan D. Olarte about the inoculation in coffee.
+
: Print electroporation protocol.
-
      Minipreps for confirmation of 1, 3 and 4.
+
: Ask Juan D. Olarte about the inoculation in coffee.
-
      Competent cells for clons 2 and 5.
+
: Minipreps for confirmation of 1, 3 and 4.
 +
: Competent cells for clons 2 and 5.
 +
</blockquote>
===July 8===
===July 8===
* Minipreps for 1, 3 and 4 were made.
* Minipreps for 1, 3 and 4 were made.
* Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
* Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
-
      Task:
+
<blockquote class="Task">
-
      To finish the minipreps from the addition of RNAsa.
+
: Task:
-
      Check the E. Coli growth in coffee.
+
: To finish the minipreps from the addition of RNAsa.
 +
: Check the E. Coli growth in coffee.
 +
</blockquote>
===July 11===
===July 11===
* Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
* Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
* Transformation protocol in chemical cells.
* Transformation protocol in chemical cells.
-
      Task:
+
<blockquote class="Task">
-
      Prepare 250 mL of SOC medium
+
: Task:
-
      Print the Transformation protocol for chemical cells.
+
: Prepare 250 mL of SOC medium
 +
: Print the Transformation protocol for chemical cells.
 +
</blockquote>
===July 12===
===July 12===
* Strains 1 and 4 have been conserved.
* Strains 1 and 4 have been conserved.
* LB liquid culture was prepared again for brick 3.
* LB liquid culture was prepared again for brick 3.
* E. Coli growth results.
* E. Coli growth results.
-
      Task:
+
<blockquote class="Task">
-
      Print the Transformation protocol for chemical cells.
+
: Task:
-
      Competent cells for clons 2 and 5.
+
: Print the Transformation protocol for chemical cells.
-
      Preserve brick 3.
+
: Competent cells for clons 2 and 5.
-
      Confirm bricks 1, 3 and 4 (Digestion)
+
: Preserve brick 3.
-
      Resuspend all Biobricks.
+
: Confirm bricks 1, 3 and 4 (Digestion)
-
      Check the E. Coli growth in coffee.
+
: Resuspend all Biobricks.
 +
: Check the E. Coli growth in coffee.
 +
</blockquote>
===July 14===
===July 14===
* E. Coli didn't grow up on the sheets.
* E. Coli didn't grow up on the sheets.
Line 69: Line 77:
* Brick 3 was left to grow in liquid medium.
* Brick 3 was left to grow in liquid medium.
* Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
* Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
-
      Task:  
+
<blockquote class="Task">
-
      Print the Transformation protocol for chemical cells.
+
: Task:  
-
      Preserve brick 3.
+
: Print the Transformation protocol for chemical cells.
-
      Confirm all biobricks (Digestion)
+
: Preserve brick 3.
-
      Sting the transformed bricks.
+
: Confirm all biobricks (Digestion)
-
      Add antibiotic to the mediums made today.
+
: Sting the transformed bricks.
 +
: Add antibiotic to the mediums made today.
 +
</blockquote>
===July 15===
===July 15===
* Brick 3 was preserved in Revco.
* Brick 3 was preserved in Revco.
Line 81: Line 91:
* No colonies in brick 6.
* No colonies in brick 6.
* Bricks 7, 9 and 10 were contaminated.
* Bricks 7, 9 and 10 were contaminated.
-
      Task:
+
<blockquote class="Task">
-
      Print the chemical cells protocol.
+
: Task:
-
      Confirm all biobricks (Digestion).
+
: Print the chemical cells protocol.
-
     
+
: Confirm all biobricks (Digestion).
 +
</blockquote>
=='''August'''==
=='''August'''==

Revision as of 05:03, 16 August 2011

HeadCol.jpg

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Contents

iGemColombia Notebook

Here you can find our daily work in the Lab!

June

June 30:

  • Biobricks 1, 2, 3, 4 and 5 were resuspended
  • Miniprep Solutions (I, II and III) were prepared.

July

July 1

  • Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

  • Biobrick 2 presented no colonies.
  • Colonies from bricks 1, 3, 4 and 5 were stinged.
Task:
To make LB medium (15x25mL)

July 6

  • Biobricks 1, 3 and 4 were twice plated.
  • Brick 5 didn't grow up.
  • We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
  • LB medium was prepared (15x25mL).
  • Liquid LB was prepared (400mL).
Task:
To add ampiciline and tetracicline to the boxes.
Claim the liquid LB
Scrape ....
Sting biobricks 2 and 5 (no clons)
Pick up the tubes with Merceditas

July 7

  • Clons 2 and 5 didn't work out.
  • Clons 1, 3 and 4 were planted in LB liquid.
  • E. Coli inoculation in Coffee
  • Kanamicine resistence plasmid: 0.2 optic density.
  • Direct inoculation x 2 and C(-) MgCl2.
Task:
Print electroporation protocol.
Ask Juan D. Olarte about the inoculation in coffee.
Minipreps for confirmation of 1, 3 and 4.
Competent cells for clons 2 and 5.

July 8

  • Minipreps for 1, 3 and 4 were made.
  • Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
Task:
To finish the minipreps from the addition of RNAsa.
Check the E. Coli growth in coffee.

July 11

  • Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
  • Transformation protocol in chemical cells.
Task:
Prepare 250 mL of SOC medium
Print the Transformation protocol for chemical cells.

July 12

  • Strains 1 and 4 have been conserved.
  • LB liquid culture was prepared again for brick 3.
  • E. Coli growth results.
Task:
Print the Transformation protocol for chemical cells.
Competent cells for clons 2 and 5.
Preserve brick 3.
Confirm bricks 1, 3 and 4 (Digestion)
Resuspend all Biobricks.
Check the E. Coli growth in coffee.

July 14

  • E. Coli didn't grow up on the sheets.
  • Chemical competent cells were made (DH5α).
  • Brick 3 was left to grow in liquid medium.
  • Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
Task:
Print the Transformation protocol for chemical cells.
Preserve brick 3.
Confirm all biobricks (Digestion)
Sting the transformed bricks.
Add antibiotic to the mediums made today.

July 15

  • Brick 3 was preserved in Revco.
  • Bricks 2, 5 and 8 were stinged.
  • 25 LB+Kan boxes x 25 mL.
  • No colonies in brick 6.
  • Bricks 7, 9 and 10 were contaminated.
Task:
Print the chemical cells protocol.
Confirm all biobricks (Digestion).

August

Retrieved from "http://2011.igem.org/Team:Colombia/Notebook"