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Team:Colombia/Notebook
From 2011.igem.org
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* Biobricks 1, 3 and 4 were twice plated. | * Biobricks 1, 3 and 4 were twice plated. | ||
* Brick 5 didn't grow up. | * Brick 5 didn't grow up. | ||
| - | * We've added Ampiciline (3.75mL)and Kanamicine (1.875mL)to the boxed from the previous day. | + | * We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day. |
* LB medium was prepared (15x25mL). | * LB medium was prepared (15x25mL). | ||
* Liquid LB was prepared (400mL). | * Liquid LB was prepared (400mL). | ||
Revision as of 03:51, 16 August 2011
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Contents |
Daily iGemWork
Here you can find our daily work in the Lab!
June
June 30:
- Biobricks 1, 2, 3, 4 and 5 were resuspended
- Miniprep Solutions (I, II and III) were prepared.
July
July 1
- Biobricks 1, 2, 3, 4 and 5 were resuspended.
July 5
- Biobrick 2 presented no colonies.
- Colonies from bricks 1, 3, 4 and 5 were stinged.
Task: To make LB medium (15x25mL)
July 6
- Biobricks 1, 3 and 4 were twice plated.
- Brick 5 didn't grow up.
- We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
- LB medium was prepared (15x25mL).
- Liquid LB was prepared (400mL).
Task: To add ampiciline and tetracicline to the boxes.
Claim the liquid LB
Scrape ....
Sting biobricks 2 and 5 (no clons)
Pick up the tubes with Merceditas
