Team:Cornell/Week 11

From 2011.igem.org

(Difference between revisions)
(Monday, August 15)
(Monday, August 15)
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==Monday, August 15==
==Monday, August 15==
-
Afternoon lab work done by: Youjin Cho and Maneesh Gupta
+
Afternoon lab work done by: Youjin Cho, Maneesh Gupta
-
:'''Obejective'''
+
:'''Objective'''
-
::*Miniprep GFP samples that were cultured overnight and send them off to sequencing.
+
::*Miniprep GFP samples that were cultured overnight and send them off for sequencing.
::*Set-up the PCR for vioB using vio operon.
::*Set-up the PCR for vioB using vio operon.
:'''Miniprep & Sequencing'''
:'''Miniprep & Sequencing'''
::*Miniprepped the GFP samples using standard Qiagen Miniprep protocol.
::*Miniprepped the GFP samples using standard Qiagen Miniprep protocol.
-
:::GFP+Avitag+pZE12 backbone (1)- 1,2,3
+
:::GFP+Avi-Tag+pZE12 backbone (1)- 1,2,3
-
:::GFP+Avitag+pZE12 backbone (2)- 1,2,3
+
:::GFP+Avi-Tag+pZE12 backbone (2)- 1,2,3
::*Set-up the samples for sequencing using the reverse primer.
::*Set-up the samples for sequencing using the reverse primer.
:::Order number: 10254977
:::Order number: 10254977
-
:'''PCR reaction'''
+
:'''PCR Reaction'''
::*Set-up the vioB PCR using the vio operon (=20.3ng/μL).
::*Set-up the vioB PCR using the vio operon (=20.3ng/μL).
::*As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes.
::*As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes.

Revision as of 20:11, 15 August 2011

Results | Protocol | Notebook | Parts Submitted

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


August 14th - August 20th

Sunday, August 14

Afternoon lab work done by: Charlie Chung

  • Picked three colonies from the two (GFP + Avi-Tagged pZE12 backbone) plates
- GFP + Avi + BB (1) #1, 2, 3
- GFP + Avi + BB (2) #1, 2, 3
  • Incubating overnight in 37°C shaker of Room 304
  • Re-picked three colonies from the (RFP + Avi-Tagged pZE12 backbone) plate in order to retry subculturing, induction, and cell lysis
- RFP + Avi + BB #7, 8, 9
  • Incubating overnight in 37°C shaker of Room 304

Monday, August 15

Afternoon lab work done by: Youjin Cho, Maneesh Gupta

Objective
  • Miniprep GFP samples that were cultured overnight and send them off for sequencing.
  • Set-up the PCR for vioB using vio operon.
Miniprep & Sequencing
  • Miniprepped the GFP samples using standard Qiagen Miniprep protocol.
GFP+Avi-Tag+pZE12 backbone (1)- 1,2,3
GFP+Avi-Tag+pZE12 backbone (2)- 1,2,3
  • Set-up the samples for sequencing using the reverse primer.
Order number: 10254977
PCR Reaction
  • Set-up the vioB PCR using the vio operon (=20.3ng/μL).
  • As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes.

Tuesday, August 16

Wednesday, August 17

Thursday, August 18

Friday, August 19

Saturday, August 20