Team:UT Dallas/NotebookWeek3

From 2011.igem.org

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           <h2><span> Week 3</span></h2>
           <h2><span> Week 3</span></h2>
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           <p><font size = "5" face= "arial" color = "#184D79"><b>July 25-</b></font>
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              <blockquote><li type = "circle">Incubate LB chlora for 1 hour<li type = "circle">Negative control continued overnight<li type = "circle">Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 28-</b></font>
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              <blockquote><li type = "circle">i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate <li type = "circle">Incubation FOFR colonies (3 mL of LB and carb)<li type = "circle">Plating mutated CheZ (inoculation)<li type = "circle">grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB<li type = "circle">-grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 29-</b></font>
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              <blockquote><li type = "circle">Making glycerol stock<li type = "circle">Miniprep<li type = "circle">Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow<li type = "circle">Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity</blockquote>
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        <p><font size = "5" face= "arial" color = "#184D79"><b>July 31-</b></font>
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              <blockquote><li type = "circle">Incubation of CheZ (I2IT)- 3mL of LB and carb</blockquote>
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Revision as of 18:43, 15 August 2011

biz solution

Week 3

July 25-

  • Incubate LB chlora for 1 hour
  • Negative control continued overnight
  • Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.

    • July 28-

  • i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate
  • Incubation FOFR colonies (3 mL of LB and carb)
  • Plating mutated CheZ (inoculation)
  • grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB
  • -grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB

    • July 29-

  • Making glycerol stock
  • Miniprep
  • Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow
  • Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity

    • July 31-

  • Incubation of CheZ (I2IT)- 3mL of LB and carb

    • Weeks 1-12

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