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Solution II
From 2011.igem.org
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(Making Solution II for Plasmid Protein Purification) |
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The ideal Solution II would be freshly made, using 10 N NaOH. | The ideal Solution II would be freshly made, using 10 N NaOH. | ||
| - | To make 5 ml of Solution II using 0.4 | + | To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use: |
| - | 2.50 ml 0.4 | + | 2.50 ml 0.4 N NaOH |
0.05 ml 10% SDS | 0.05 ml 10% SDS | ||
| - | 2.45 ml distilled water or NF water | + | 2.45 ml distilled water or [[NF water]] |
| + | |||
| + | Notice 0.05 ml = 50 ul of the 10% SDS. | ||
Latest revision as of 02:39, 15 August 2011
0.2 N NaOH [Think M when you see N] 1.0% SDS
The ideal Solution II would be freshly made, using 10 N NaOH.
To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:
2.50 ml 0.4 N NaOH 0.05 ml 10% SDS 2.45 ml distilled water or NF water
Notice 0.05 ml = 50 ul of the 10% SDS.
