"
Solution II
From 2011.igem.org
(Difference between revisions)
(Created page with "0.2 M NaOH 1% SDS") |
|||
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | 0.2 | + | |
| - | 1% | + | 0.2 N NaOH [Think M when you see N] |
| + | 1.0% SDS | ||
| + | |||
| + | The ideal Solution II would be freshly made, using 10 N NaOH. | ||
| + | |||
| + | To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use: | ||
| + | 2.50 ml 0.4 N NaOH | ||
| + | 0.05 ml 10% SDS | ||
| + | 2.45 ml distilled water or [[NF water]] | ||
| + | |||
| + | Notice 0.05 ml = 50 ul of the 10% SDS. | ||
Latest revision as of 02:39, 15 August 2011
0.2 N NaOH [Think M when you see N] 1.0% SDS
The ideal Solution II would be freshly made, using 10 N NaOH.
To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:
2.50 ml 0.4 N NaOH 0.05 ml 10% SDS 2.45 ml distilled water or NF water
Notice 0.05 ml = 50 ul of the 10% SDS.
