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Solution I
From 2011.igem.org
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| - | 50 mM glucose | + | 50 mM glucose Link to [[50 mM glucose]] |
| - | 25 mM Tris Cl (pH 8.0) | + | 25 mM Tris Cl (pH 8.0) |
| - | 10 mM EDTA (pH 8.0) | + | 10 mM EDTA (pH 8.0) |
Solution I can be prepared in batches of approximately 100 ml, autoclaved for 15 minutes at 10lb/sq. in. on liquid cycle, and stored at 4 degrees C | Solution I can be prepared in batches of approximately 100 ml, autoclaved for 15 minutes at 10lb/sq. in. on liquid cycle, and stored at 4 degrees C | ||
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| + | This is 1 of 3 solutions that one can use in a process to purify plasmid DNA. The advantage of using Solutions I, II and III is that the reagents required to make these solutions are readily available - most of what was used to make these solutions was donated by colleagues who had the compounds in pure, powdered form. Because these same colleagues have money for things like "quick" spin columns for DNA purification, many of the ingredients were simply collecting dust in a cabinet. | ||
Latest revision as of 02:21, 15 August 2011
50 mM glucose Link to 50 mM glucose 25 mM Tris Cl (pH 8.0) 10 mM EDTA (pH 8.0)
Solution I can be prepared in batches of approximately 100 ml, autoclaved for 15 minutes at 10lb/sq. in. on liquid cycle, and stored at 4 degrees C
This is 1 of 3 solutions that one can use in a process to purify plasmid DNA. The advantage of using Solutions I, II and III is that the reagents required to make these solutions are readily available - most of what was used to make these solutions was donated by colleagues who had the compounds in pure, powdered form. Because these same colleagues have money for things like "quick" spin columns for DNA purification, many of the ingredients were simply collecting dust in a cabinet.
