Team:British Columbia/Protocols/Peace

From 2011.igem.org

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'''Our iGEM team safety rules that everyone on the team has agreed to follow:'''
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*We have a 8am - 9pm work hours curfew for our iGEM laboratory work
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*iGEM students will never work alone in the laboratory, you'll always have someone else there with you. This is for safety.
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*The iGEM supervisors are Alina Chan and Rafael Saer. If you are ever in need of a supervisor and you can not reach them, you should come find Dr. Joanne Fox.
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*We also have contact information for all team members in our safety binder which is located on the iGEM lab bench.  *Our Ellis lab contact is: Albert Cairo; Our Bohlmann lab contact is: Karen Reid.

Revision as of 19:03, 13 August 2011

Team: British Columbia - 2011.igem.org

iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.

Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.


Our iGEM team safety rules that everyone on the team has agreed to follow:

  • We have a 8am - 9pm work hours curfew for our iGEM laboratory work
  • iGEM students will never work alone in the laboratory, you'll always have someone else there with you. This is for safety.
  • The iGEM supervisors are Alina Chan and Rafael Saer. If you are ever in need of a supervisor and you can not reach them, you should come find Dr. Joanne Fox.
  • We also have contact information for all team members in our safety binder which is located on the iGEM lab bench. *Our Ellis lab contact is: Albert Cairo; Our Bohlmann lab contact is: Karen Reid.