"
Team:Paris Liliane Bettencourt/Notebook/2011/08/13/
From 2011.igem.org
(Difference between revisions)
(Created page with "== Cyrille == === Adding the RFP biobrick in pHM3 === We want to insert the biobrick J04450 into pHM3 for three reasons: * To have a negative control of digestion * To restore...") |
(→Adding the RFP biobrick in pHM3) |
||
| Line 9: | Line 9: | ||
* To test the possibility of cloning in this plasmid | * To test the possibility of cloning in this plasmid | ||
| - | 3 times 500 ng of the pHM3 plasmid is digested in E P for | + | 3 times 500 ng of the pHM3 plasmid is digested in E P for 30 min |
| - | 3 times 500ng of J04450 in pSB1C3 is digested using E P sites. The result is loaded on a gel and will undergo a gel purification. | + | 3 times 500ng of J04450 in pSB1C3 is digested using E P sites. |
| + | |||
| + | The result is loaded on a gel. The and will undergo a gel purification. | ||
| + | [[File:CP1311_gelextr_S27.jpg|thumb|center|Insert digested before cutting the bands]] | ||
| + | [[File:CP1311_gelextr_S27_cut.jpg|thumb|center|Insert digested after cutting the bands]] | ||
| + | |||
| + | [[File:CP1311_gelextr_pHM3.jpg|thumb|center|plasmid digested before cutting the bands]] | ||
| + | [[File:CP1311_gelextr_phm3_cut.jpg|thumb|center|plasmid digested after cutting the bands]] | ||
Revision as of 11:31, 13 August 2011
Cyrille
Adding the RFP biobrick in pHM3
We want to insert the biobrick J04450 into pHM3 for three reasons:
- To have a negative control of digestion
- To restore the EXSP biobrick format in the plasmid
- To test the possibility of cloning in this plasmid
3 times 500 ng of the pHM3 plasmid is digested in E P for 30 min
3 times 500ng of J04450 in pSB1C3 is digested using E P sites.
The result is loaded on a gel. The and will undergo a gel purification.
