"
Team:Northwestern/Notebook/Protocols/CCMB80
From 2011.igem.org
(Difference between revisions)
Helenmelon (Talk | contribs) (Created page with "__NOTOC__ {{:Team:Northwestern/Templates/trial}} <!--Week 1--> <DIV style="font-size:20px"> CCMB80 Buffer </DIV> ---------------------- *10 mM KOAc pH 7.0 (10 ml of a 1M stock/...") |
Helenmelon (Talk | contribs) |
||
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
{{:Team:Northwestern/Templates/trial}} | {{:Team:Northwestern/Templates/trial}} | ||
<!--Week 1--> | <!--Week 1--> | ||
| + | |||
<DIV style="font-size:20px"> | <DIV style="font-size:20px"> | ||
| Line 7: | Line 8: | ||
</DIV> | </DIV> | ||
---------------------- | ---------------------- | ||
| + | |||
*10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) | *10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) | ||
| Line 17: | Line 19: | ||
*Sterile filter and store at 4°C | *Sterile filter and store at 4°C | ||
*Slight dark precipitate appears not to affect its function | *Slight dark precipitate appears not to affect its function | ||
| + | |||
Adapted from [http://openwetware.org/wiki/TOP10_chemically_competent_cells OpenWetWare] | Adapted from [http://openwetware.org/wiki/TOP10_chemically_competent_cells OpenWetWare] | ||
Latest revision as of 18:14, 12 August 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
CCMB80 Buffer
- 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L)
- 80 mM CaCl2.2H2O (11.8 g/L)
- 20 mM MnCl2.4H2O (4.0 g/L)
- 10 mM MgCl2.6H2O (2.0 g/L)
- 10% glycerol (100 ml/L)
- Adjust pH DOWN to 6.4 with 0.1N HCl if necessary
- Adjusting pH up will precipitate manganese dioxide from Mn containing solutions.
- Sterile filter and store at 4°C
- Slight dark precipitate appears not to affect its function
Adapted from [http://openwetware.org/wiki/TOP10_chemically_competent_cells OpenWetWare]
