Team:Osaka/week1

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Revision as of 13:50, 11 August 2011

August 1 (Mon)

1. Preparation of LB agar plates (33 Amp, 18 Kan, 17 Cam).

August 2 (Tue)

1. Culture medium preparation
2. Competent cells preparation - Nojima Method
   • SOB medium (MgCl2 not yet added) -> stored at 4˚C
   • Preparation of glucose solution for making SOC medium.
   • Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n

Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.

1. Transformation of Registry parts (See Table 1).

Note: 'ID' is an internal identifier used by wet work members to simplify labeling etc. In the case of iGEM distribution parts it usually refers to the plate location.

August 3 (Wed)

1. Competent cells preparation (continued)
   • Inoculation of pre-culture SOB from o/n-incubated agar plate colonies.
   • Transfer from pre-culture to growth culture.
2. Colony check
   • All parts successfully transformed
3. Transfer to LB culture medium

August 4 (Thu)

1. OD measurements throughout the day till required OD (0.3~0.7) was obtained.
2. Completion of competent cells according to protocol.
3. Miniprep of 1-9N,1-2M,1-14K,1-12M,1-1D,1-17P,1-1G,1-5A,2-16,2-18H,2-16N,1-3A,3-6H

August 5 (Fri)

August 6 (Sat)

1. Transformation of Registry parts (See Table 1).

Note: 'ID' is an internal identifier used by wet work members to simplify labeling etc. In the case of iGEM distribution parts it usually refers to the plate location.

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