Team:Cornell/Week 1

From 2011.igem.org

(Difference between revisions)
(Tuesday)
(Tuesday)
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==Tuesday==
==Tuesday==
:'''CUgem bootcamp training session (day 1)'''
:'''CUgem bootcamp training session (day 1)'''
-
::'''PartI'''
+
::'''Part I'''
:::1. PCR setup
:::1. PCR setup
:::2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
:::2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
Line 19: Line 19:
:::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
:::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
:::5. PCR clean up the samples and quantify using nanodrop.
:::5. PCR clean up the samples and quantify using nanodrop.
-
::'''PartII'''
+
::'''Part II'''
:::1. Miniprepping the overnight culture using Qaigen Kit.
:::1. Miniprepping the overnight culture using Qaigen Kit.
:::2. Quantify the samples using nanodrop.
:::2. Quantify the samples using nanodrop.

Revision as of 17:26, 10 August 2011

Results | Protocol | Notebook | Parts Submitted

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


June 5th - June 11th

Sunday

Monday

Tuesday

CUgem bootcamp training session (day 1)
Part I
1. PCR setup
2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
3. Quantify the samples using nanodrop.
4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
5. PCR clean up the samples and quantify using nanodrop.
Part II
1. Miniprepping the overnight culture using Qaigen Kit.
2. Quantify the samples using nanodrop.
3. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes.
5. Run the samples on the agarose gel.
6. Cut out the band of right size, gel purify and quantify with nanodrop.

Wednesday

Weill Hall labspace introduction by Dr.Archer
Cugem bootcamp training session (day 2)

Thursday

Cugem bootcamp training session (day 3)

Friday

Team meeting
  • Organized the new lab space in Weill.
  • Ordered the materials needed for the labwork.

Saturday