Team:Caltech/Week 9

August 8

Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet
PCR of 16s
Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.
Re-assembly of DDT gene using alternate method
GCMS of p450-degraded BPA samples Obtain experimental strain (biofilm-forming) MG-1655 E. coli and grow overnight in SOC

Results

Bacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well.

96-well plate with XL-10 biofilm grown for two days, visualized with 1% crystal violet

Glass beads with XL-10 biofilm grown for two days, visualized with 1% crystal violet

Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands.
BPA sample was too diluted for GCMS; must concentrate first.

August 9

Evaporate-centrifuge BPA sample to concentrate for GCMS Create glycerol stocks of experimental E. coli strain MG-1655 for biofilm experiments Did 16s sequencing experiments with a Master Mix.