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Team:Caltech/Week 9
From 2011.igem.org
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Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.<br/> | Transformation of I732019, I732005, J23119 from source plate in XL-10 gold.<br/> | ||
Re-assembly of DDT gene using alternate method<br/> | Re-assembly of DDT gene using alternate method<br/> | ||
| - | GCMS of p450-degraded BPA | + | GCMS of p450-degraded BPA samples |
| + | Obtain experimental strain (biofilm-forming) MG-1655 ''E. coli'' and grow overnight in SOC | ||
===Results=== | ===Results=== | ||
Bacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well. | Bacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well. | ||
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BPA sample was too diluted for GCMS; must concentrate first. | BPA sample was too diluted for GCMS; must concentrate first. | ||
==August 9== | ==August 9== | ||
| - | Evaporate-centrifuge BPA sample to concentrate | + | Evaporate-centrifuge BPA sample to concentrate for GCMS |
| + | Create glycerol stocks of experimental ''E. coli'' strain MG-1655 for biofilm experiments | ||
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Revision as of 21:56, 9 August 2011
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Project |
August 8Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet ResultsBacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well.
Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands. August 9Evaporate-centrifuge BPA sample to concentrate for GCMS Create glycerol stocks of experimental E. coli strain MG-1655 for biofilm experiments
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