From 2011.igem.org
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| - | <h2> Grenoble Project</h2>
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| - | <img src="https://static.igem.org/mediawiki/2011/3/38/Jb.png" class="icon"/>
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| - | <h2> Abstract<span>JB</span></h2>
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| - | Only ranges of pollutant concentration have been measured in the iGEM competition until now. Our goal is to create a simple and accurate, ready-to-use bioquantifier for heavy metals. Simple enough for an easy visual checking and a routine use in the labs. Our system is inspired from Gardner's work on toggle switch biosystem and from former iGem teams' works on quorum sensing communication.</p>
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| - | Our purpose is to add a pollutant to a device containing a bacterial layer on an engineered medium. This engineered medium is an IPTG gradient.Add of that pollutant to our system induces a geographical division of the plate into two areas : one where the IPTG concentration prevails, and the other where the pollutant concentration prevails. Toggle switch is a biological network which allows to lock our biosystem in a specific state. We use it here in association with complementary quorum sensing genes : A sender and a receiver. Thus, at the interface between both, the reception of quorum sensing molecules by the receptors will induce the coloration of the bacteria. Finally we will obtain a colored line from which we can get the unknown concentration of pollutant.</p>
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| - | Up to now the quantification of heavy metals requires complex physical and chemical protocols. We propose a new way of quantifying heavy metals, much easier than these ones. A first step to a pure water for everyone!</p>
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Revision as of 14:34, 8 August 2011
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