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Team:Cornell/Week 10
From 2011.igem.org
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==Sunday== | ==Sunday== | ||
| + | Morning lab work done by: Youjin Cho and Claire Paduano | ||
| + | |||
| + | :'''Objective''' | ||
| + | ::Prepare VioE in Avi-Tagged pZE12 vector backbone for VioA and VioB gene inserts | ||
| + | :'''Digestion Setup''' | ||
| + | :::23.65μL H2O | ||
| + | :::18.6μL VioE in Avi-Tagged pZE12 vector backbone (1μg) | ||
| + | :::5μL 10x NEBuffer 2 | ||
| + | :::0.5μL 100x BSA | ||
| + | :::1.25μL KpnI | ||
| + | :::<u>1μL HindIII</u> | ||
| + | :::50μL Total | ||
| + | ::KpnI has only 75% efficiency in Buffer 2, so added 0.25uL more KpnI to digestion mixture | ||
| + | ::Incubate in 37°C water bath for 2 hours | ||
==Monday== | ==Monday== | ||
Revision as of 18:37, 7 August 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
August 7th - August 13th
Sunday
Morning lab work done by: Youjin Cho and Claire Paduano
- Objective
- Prepare VioE in Avi-Tagged pZE12 vector backbone for VioA and VioB gene inserts
- Digestion Setup
- 23.65μL H2O
- 18.6μL VioE in Avi-Tagged pZE12 vector backbone (1μg)
- 5μL 10x NEBuffer 2
- 0.5μL 100x BSA
- 1.25μL KpnI
- 1μL HindIII
- 50μL Total
- KpnI has only 75% efficiency in Buffer 2, so added 0.25uL more KpnI to digestion mixture
- Incubate in 37°C water bath for 2 hours
